Angiotensin II (Ang II) elicits an Ang II type 2 (AT2) receptor-mediated increase in delayed-rectifier K+ current (IK) in neurons cultured from newborn rat hypothalamus and brainstem. This effect involves a pertussis toxin (PTX)-sensitive Gi protein and is abolished by inhibition of serine and threonine phosphatase 2A (PP-2A). Here, we determined that Ang II stimulates [3H]arachidonic acid (AA)...

In two experiments we evaluated the effect of water and acetic acid soaking on ME, apparent amino acid (AA) availability, and true AA availability of vetch (V) and ervil (E) seeds. In Experiment 1, the feedstuffs were untreated (U) V or coarsely ground V soaked in water (1:10, wt/vol) at 40 C for 72 h with a water change every 12 h (40WV), vetch soaked in 1% acetic acid for 24 h at 40 C (40AAV)...

Functionalized chiral diazaphospholanes ligate to a variety of transition metals, yielding chiral, catalytically active, metal complexes. Previous work has established that amino acid derivatization of the carboxyl groups of (R,R)-N,N'-phthaloyl-2,3-(2-carboxyphenyl)-phenyl-3,4-diazaphospholane (1) yields phosphines that are excellent ligands for palladium-catalyzed asymmetric allylic alkylatio...

Hepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this study, the binding sites between E2 and aptamer ZE2 were further explored. E2 was truncated to 15...

Ileal digesta were collected via a post-valve-T-caecum cannula from piglets ( lo-15 kg) fed diets based on raw pea or on pea protein isolate (Finale or Frijaune variety). From the determined amino acids (AA) content of feed and digesta, AA apparent digestibility coefficients and proportions of proteins (endogenous, bacterial, dietary) theoretically present in digesta were calculated. Four essen...

The purpose of this study was to look at the effects of an amino-acid mixture, highfat feeding and exercise on blood glucose and plasma insulin levels in skeletal muscle of female Sprague-Dawley rats. Rats were either fed a standard-diet only (SD-CON), a standard-diet plus a pre-established amino-acid mixture (CON-AA), a high-fat diet only (HF-CON), a high-fat diet plus a pre-established amino-...

background: dermatophagoides farinae and dermatophagoides pteronyssinus are house dust mites (hdm) that they cause severe asthma and allergic symptoms. tropomyosin protein plays an important role in mentioned immune and allergic reactions to hdms. here, tropomyosin protein from dermatophagoides spp. was comprehensively screened in silico for its allergenicity, antigenicity and similarity/conser...

Transcription factor IIF (TFIIF) cooperates with RNA polymerase II (pol II) during multiple stages of the transcription cycle including preinitiation complex assembly, initiation, elongation, and possibly termination and recycling. Human TFIIF appears to be an alpha2beta2 heterotetramer of RNA polymerase II-associating protein 74- and 30-kDa subunits (RAP74 and RAP30). From inspection of its 51...

The agonist-induced phosphorylation sites of the rat AT1a angiotensin receptor were analyzed using epitope-tagged mutant receptors expressed in Cos-7 cells. Angiotensin II-stimulated receptor phosphorylation was unaffected by truncation of the cytoplasmic tail of the receptor at Ser342 (Delta342) but was abolished by truncation at Ser325 (Delta325). Truncation at Ser335 (Delta335), or double-po...

Amino acid (AA) and fatty (FA) composition of the fertilised eggs different larval stages (at 0, 3, 6, 9, 12, 15 and21 days post-hatch, dph) Chanos chanos was investigated. The total indispensable amino acids (IAA) contributed to55.62% AA in egg which reduced to 52.54% on 6 dph. profile C. found be rich invaline (7.99%), leucine (7.51%) lysine (6.98%) poor histidine (2.36%) methionine (2.47%), ...