New site-specific protein labeling (SSPL) reactions for targeting-specific, short peptides could be useful for the real-time detection of proteins inside of living cells. One SSPL approach matches bioorthogonal reagents with complementary peptides. Here, hydrazide reactive peptides were selected from phage-displayed libraries using reaction-based selections. Selection conditions included washes...

This study reports the anti-yeast effect of the 4-methoxybenzoic acid hydrazide (pmbah), 4-chlorobenzoic acid hydrazide (pcbah) and their Pt(II) complexes: cis- [PtL2X2] and cis- [PtL(NH3)Cl2] where L is either pcbah or pmbah and X is Cl, Br or I. MICs of the 4- substituted analogues (20,000-625 microM) are much lower than those of the previously reported benzoic acid hydrazide and 3-methoxyben...

Methyl 3,4,5-trihydroxybenzoate (methyl gallate) was synthesized from 3,4,5-trihydroxybenzoic acid (gallic acid) by reaction with methanol in presence of sulphuric acid. gallate then reacted hydrazine monohydrate to give 3,4,5-trihydroxybenzohydrazide (galloyl hydrazide). The structure methyl and galloyl hydrazide characterized Fourier transforms infrared (FTIR) spectroscopy, mass spectrometry ...

A convenient synthesis of (S)-proline hydrazide 2a via the reaction of ethyl (S)-N-benzylprolinate with hydrazine hydrate and subsequent deprotection of (S)-N-benzyl proline hydrazide 5 is described. The latter, in methanolic solution, reacted with aromatic aldehydes as well as with cycloaliphatic ketones at room temperature to give the corresponding hydrazones of type 7 in good yields. The str...

In the present study, oligo(ethylene glycol) (OEG)-linked alkanethiols were synthesized which carry a vicinal diol on one end of the OEG chain. After self-assembled monolayer (SAM) formation on gold, the vicinal diols were converted into aldehyde functions by exposure to aqueous NaIO4, as previously used for SAMs with OEG chains buried in the center of the SAM [Jang et al. Nano Lett. 2003, 3, 6...

Rat liver glutamine transaminase acts on y-glutamyl hydrazones of a-keto acids to yield the corresponding L-amino acids and 3-hydroxy-tetrahydro-6-pyridazinone-3-carboxyllc acid; the latter compound undergoes nonenzymatic dehydration in acid to yield 1,4,5,6-tetrahydro-6-pyridazinone -3carboxylic acid. L-Amino acid oxidase (snake venom) also catalyzes the formation of 3-hydroxy-tetrahydro-6-pyr...

An enzymatic system in rat liver microsomal preparations has been detected that catalyzes the transfer of the 2'-phospho-AMP moiety from NADP to endogenous polypeptides; the major acceptor is a polypeptide of about 40 kDa (p40). Modification of the acceptor by 2'-phospho-AMP residues was deduced from the simultaneous transfer of 2'-[33P]phosphate and [3H]adenine residues from double-labeled NAD...