Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64% +/- 7% of the mononuclear cells in 600 ml of peripheral blood were collected in a 30-ml volume. Analysis of sequential 5-ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenit...

The c-fms gene product is related, and possibly identical, to the receptor for the mononuclear phagocyte colony stimulating factor, CSF-1. Using antisera to a recombinant v-fms--coded polypeptide, glycoproteins encoded by the human c-fms locus were detected in mononuclear cells from normal peripheral blood and in promyelocytic HL-60 cells 24 h after induction of monocytic differentiation with p...

new blood forming vessels are produced by differentiation of mesodermal precursor cells to angioblasts that become endothelial cells (ecs) which in turn give rise to primitive capillary network. human cord blood (hcb) contains large subsets of mononuclear cells (mncs) that can be differentiated into endothelial-like cells in vitro. human mononuclear progenitor cells were purified from fresh umb...

glioblastoma multiforme and other malignant gliomas continue to be associated with a dismal prognosis despite advances in diagnosis and therapy.1,2 Mean survival from the time of diagnosis is less than one year in most cases. This has spurred investigation of new treatment modalities such as immunogene ABSTRACT: Background: Cancer immunogene therapy is based on vaccination with radiated, autolo...

Background & Aims: Verapamil as a calcium channel blocker has been broadly used in the treatment of many cardiovascular diseases such as hypertension and arrhythmia. Furthermore, the anti-tumor/ antiinflammatory effects of verapamil have been shown. In the present study, the cytotoxic effect of verapamil on human peripheral blood mononuclear cells (PBMCs) and BALB/c peritoneal macrophges has be...

background: the major immuno-modulating effects of ganoderma lucidum include mitogenicity and activation of immune effector cells such as t cells, macrophages and natural killer cells resulting in the production of cytokines. objective: the purpose of this study was to evaluate the expression of cd40 and cd80 by g. lucidum-treated human peripheral blood mononuclear cells. methods: monocytes wer...