نتایج جستجو برای: human antibody library

تعداد نتایج: 1863887  

Journal: :BMC Biotechnology 2008
Martina Inga Kirsch Birgit Hülseweh Christoph Nacke Torsten Rülker Thomas Schirrmann Hans-Jürgen Marschall Michael Hust Stefan Dübel

BACKGROUND Venezuelan equine encephalitis virus (VEEV) belongs to the Alphavirus group. Several species of this family are also pathogenic to humans and are recognized as potential agents of biological warfare and terrorism. The objective of this work was the generation of recombinant antibodies for the detection of VEEV after a potential bioterrorism assault or an natural outbreak of VEEV. R...

Journal: :The Journal of biological chemistry 2011
Teng Zuo Xuanling Shi Zhonghua Liu Linlin Guo Qing Zhao Tianxia Guan Xianming Pan Na Jia Wuchun Cao Boping Zhou Mark Goldin Linqi Zhang

Host antibody response is a crucial defense against pathogenic infection. Here, we report a novel technique allowing quantitative measurement of polyclonal antibody response in vivo. This involves expression of a combinatorial library of target proteins from a candidate pathogen on the surface of yeast Saccharomyces cerevisiae. After mixing with serum/plasma from infected or immunized subjects,...

2007
Charlotte G. Jakobsen Nicolaj Rasmussen Anne-Vibeke Laenkholm Henrik J. Ditzel

Clinical trials using monoclonal antibodies (mAb) against cell-surface markers have yielded encouraging therapeutic results in several cancer types. Generally, however, anticancer antibodies are only efficient against a subpopulation of cancers, and there is a strong need for identification of novel targets and human antibodies against them. We have isolated single-chain human mAbs from a large...

Journal: :Nature Communications 2021

Abstract Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting receptor binding domain (RBD) of viral spike protein via antibody library constructed from peripheral blood mononuclear cells a convalescent patient. The CT-P59 mAb potently neutralizes SARS-Co...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2014
Uri Laserson Francois Vigneault Daniel Gadala-Maria Gur Yaari Mohamed Uduman Jason A Vander Heiden William Kelton Sang Taek Jung Yi Liu Jonathan Laserson Raj Chari Je-Hyuk Lee Ido Bachelet Brendan Hickey Erez Lieberman-Aiden Bozena Hanczaruk Birgitte B Simen Michael Egholm Daphne Koller George Georgiou Steven H Kleinstein George M Church

The adaptive immune system confers protection by generating a diverse repertoire of antibody receptors that are rapidly expanded and contracted in response to specific targets. Next-generation DNA sequencing now provides the opportunity to survey this complex and vast repertoire. In the present work, we describe a set of tools for the analysis of antibody repertoires and their application to el...

Journal: :Nucleic acids research 2001
L Giovannoni F Viti L Zardi D Neri

We describe here a method, based on iterative colony filter screening, for the rapid isolation of binding specificities from a large synthetic repertoire of human antibody fragments in single-chain Fv configuration. Escherichia coli cells, expressing the library of antibody fragments, are grown on a porous master filter, in contact with a second filter coated with the antigen, onto which antibo...

Journal: :Cancer research 2010
Ronan Crépin Anne-Laure Goenaga Betsy Jullienne Houcine Bougherara Christine Legay Karim Benihoud James D Marks Marie-Alix Poul

The major route of iron uptake by cells occurs through transferrin receptor (TfR)-mediated endocytosis of diferric-charged plasma transferrin (holo-Tf). In this work, we pursued TfR antibodies as potential cancer therapeutics, characterizing human single-chain variable antibody fragments (scFv) specific for the human TfR isolated from a phage display library. We hypothesized that many of these ...

2009
Mark Schütte Philippe Thullier Thibaut Pelat Xenia Wezler Philip Rosenstock Dominik Hinz Martina Inga Kirsch Mike Hasenberg Ronald Frank Thomas Schirrmann Matthias Gunzer Michael Hust Stefan Dübel

BACKGROUND Aspergillus fumigatus is a common airborne fungal pathogen for humans. It frequently causes an invasive aspergillosis (IA) in immunocompromised patients with poor prognosis. Potent antifungal drugs are very expensive and cause serious adverse effects. Their correct application requires an early and specific diagnosis of IA, which is still not properly achievable. This work aims to a ...

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