The retention model in HPLC is proposed, which is based on quasichemical equilibria of the components of the chromatographic system. This model provides a better undestanding of the retention mechanism in HPLC and it can be applied for quantitative description of the dependence of retention on the nature and composition of the mobile phase. H i g h performance liquid chromatography (HPLC) is kn...

High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This ...

A high-performance liquid chromatographic (HPLC) method with UV and DAD detection for the quantitative determination of linezolid in human serum was developed in present work. Chromatography was carried out by reversed-phase technique on a RP-18 column with a mobile phase composed of 50 mM phosphate buffer and acetonitrile (76 : 26, v/v), adjusted to pH 3.5 with orthophosphoric acid. Serum samp...

BACKGROUND The potent immunosuppressant mycophenolic acid (MPA) is metabolized to an inactive glucuronide (MPAG). The extent of metabolism varies among individuals, and the MPAG formed can be hydrolyzed to MPA and can displace MPA from serum albumin, creating a potential need to monitor both MPA and MPAG. METHODS After addition of the carboxybutoxy ether of MPA (MPAC) as internal standard, MP...

wheat flour is a commodity with a high risk of aflatoxins (afs) contamination. during the bread making there are many processes that can affect the afs stability. the effect of bread making process using different yeast types on afs levels was investigated. for this purpose, standards of afs including b and gwere added to flour and then bread loaves were prepared. three types of commercially av...

raspberry waste, obtained from two cultivars, meeker (erm) and willamette (erw) was subjected to evaluation antioxidants and antitumour activities as a potential source of phenolics. some phenolic acids and flavonoids were identified and quantified by hplc. antioxidant activity was tested by measuring ability to scavenge dpph• and •oh by esr spectroscopy. ic50dpph• varied from 0.67 for erm to 0...