Conjunctiva! biopsies from two sibs with mucolipidosis III and from their clinically normal parents were examined by histochemistry and electron microscopy. Connective tissue cells were filled with single membrane-limited vacuoles which frequently contained membranous lamellar material. These findings indicate abnormal mucopolysaccharide and glycolipid accumulation in this disorder and suggest ...

Cerny, L.: Histopathology and Histochemistry of Yersin Type Tuberculosis in Rabbits. Development of the Disease after intravenous Infection with Mycobacterium avium. Acta vet. Bmo, 48, 1979: 45-52. After intravenous aplication to rabbits of Mycobacterium avium the first morphological changes appeared 48 hours post infection. The most characteristic morphological feature of the infection was a s...

In the last three years, more than 70,000 scientific articles have been published in peer reviewed journals on the application of histochemistry in the biomedical field: most of them did not appear in strictly histochemical journals, but in others dealing with cell and molecular biology, medicine or biotechnology. This proves that histochemistry is still an active and innovative discipline with...

Cholinergic muscarinic receptor binding and acetylcholinesterase (AChE) histochemistry were studied in the barrel cortex of adult, vibrissae deprived and vibrissae denervated mice. In the control barrel field muscarinic receptors labeled with [3H]quinuclidinyl benzilate ([3H]QNB) showed a higher density in the granular cortex and a higher accumulation of label in the barrels. AChE staining reve...

The sites of synthesis of the chromogranins A and B, and their potential processed peptides, were examined by quantitating the levels of chromogranin A and B mRNA in various regions of the human brain by Northern blot analysis. Chromogranin A and B mRNA expression in the brain is region-specific and confined to grey matter. In situ hybridization histochemistry detected chromogranin A and B mRNA...

Plant and invertebrate lectins are valuable cyto- and histological tools for the localization of defined carbohydrate determinants. The well-documented ubiquitous occurrence of sugar receptors encourages functional considerations. Undoubtedly, analysis of the presence of vertebrate lectins in tissues and cells is required to answer the pertinent and tempting question on the physiological releva...

Large, Ca(2+)-activated K(+) channels (BK), comprised of alpha- and beta-subunits, mediate K(+) secretion during high flow rates in distal nephron segments. Because the BK-beta1 subunit enhances Ca(2+) sensitivity of BK in a variety of cells, we determined its role in flow-induced K(+) secretion and its localization in the mammalian nephron. To determine the role of BK-beta1 in the kaliuretic r...

Principle of Technique In recent years considerable interest has been directed toward defining projections of neurons containing specific neurotransmitten substances. As a result, numerous techniques have been developed that use fluorescent retrograde cell markers (2,6,10,1 1,19) on horseradish peroxidase (HRP) (3,4,13,14,17) combined with the fluorescence histochemistry (2, 1 7,19), immunohist...

Previous studies have shown that in developing monkey corticostriatal fibres terminate around striatal cytoarchitectonic compartments--cell islands, showing transfiguration around 105th embryonic day (E105) of gestation. In the present study we have analyzed these striatal cytoarchitectonic islands and acetylcholinesterase (AChE) rich patches in the developing human brain considering them as st...

Vessels within the lymphocytic infiltrate in 161 examples of 24 different diseases have been studied using light and electron microscopy and histochemistry. Hitherto undescribed venules have been identified within lymphocyte aggregates which exhibit a structure, ultrastructure and cytochemistry previously believed to be restricted to the high endothelial venules of lymphoid tissues. The signifi...