OBJECTIVE To evaluate the frequency dependence of ultrasonic backscatter for its ability to differentiate between neoplastic and healthy tissue. METHODS Standard B-mode images were created of 5 rats with spontaneous mammary tumors, and regions of interest in the lesion and surrounding tissue were parameterized by the slope of the backscatter amplitude versus frequency. RESULTS In 4 of the 5...

Kazuo MIZUMOTO1), Yoshihiro NOSO1), Shigeru SAKANO2), Kanji FURUYA3) and Riruke MARUYAMA4) 1)Department of surgery, Ohda General Medicine Education Center, 1428-3 Ohda-cho, Ohda, Shimane 694-0063, Japan 2)Department of surgery, Ohda City Hospital, 1428-3 Ohda-cho, Ohda, Shimane 694-0063, Japan 3)Department of internal medicine, Ohda City Hospital, 1428-3 Ohda-cho, Ohda, Shimane 694-0063, Japan ...

We report a case of HLA B27 negative Crohn's spondylitis with a detailed study of the proband's family, including tissue types of all living relatives. The family included cases of psoriasis, psoriatic spondylitis, and idiopathic ankylosing spondylitis. We believe this to be the first report of these associated diseases occurring in one family. All relatives were B27 negative, including one wit...

The highly polymorphic and complex system of human leucocyte antigens known as HL-A has been intensively investigated in recent years.1,2 It seems to be widely understood that the research is very actively engaged at the present time in the task and prospect of tissue typing for selecting donors and recipients for organ transplantation in man. In the near future, however, one may anticipate the...

Environmental and genetic interventions extend health span in a range of organisms by triggering changes in different specific but complementary pathways. We investigated the gene expression changes that occur across species when health span is extended via different interventions. To perform this comparison using heterogeneous datasets from different measurement platforms and organisms, we dev...

BACKGROUND The accuracy of quantitative real-time PCR (qRT-PCR) is highly dependent on reliable reference gene(s). Some housekeeping genes which are commonly used for normalization are widely recognized as inappropriate in many experimental conditions. This study aimed to identify reference genes for clinical studies through microarray meta-analysis of human clinical samples. METHODOLOGY/PRIN...

There are certain genes that have ubiquitous expression patterns in an individual’s cells; yet, a paradox exists whereby mutations (germline or somatic) in such genes are only strongly associated with cancers of specific tissues. As these genes are ubiquitously expressed in the body’s cells and thereby have functions in -potentiallyall tissue types, then surely their resulting defects would man...

Meta-analyses combining gene expression microarray experiments offer new insights into the molecular pathophysiology of disease not evident from individual experiments. Although the established technical reproducibility of microarrays serves as a basis for meta-analysis, pathophysiological reproducibility across experiments is not well established. In this study, we carried out a large-scale an...

Duplicated genes can contribute to the evolution of new functions and they are common in eukaryotic genomes. After duplication, genes can show divergence in their sequence and/or expression patterns. Qualitative complementary expression, or reciprocal expression, is when only one copy is expressed in some organ or tissue types and only the other copy is expressed in others, indicative of regula...

A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the relative contributions of extracellular and intracellular fluorophores to the intrinsic fluorescence...