نتایج جستجو برای: his beloved woman
تعداد نتایج: 243129 فیلتر نتایج به سال:
Escherichia coli K1 is a leading pathogen in neonatal sepsis and meningitis. The K1 capsule, composed of alpha2,8-linked polysialic acid, represents the major virulence factor. In some K1 strains, phase-variable O-acetylation of the capsular polysaccharide is observed, a modification that is catalyzed by the prophage-encoded O-acetyltransferase NeuO. Phase variation is mediated by changes in th...
This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ...
Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested...
FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...
The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...
Via Sons and Lovers, D,H, Lawrence aims to bring about attitudinal legislative changes that enable woman achieve her rights. His work is a project refuses the Victorian stereotype of woman—the housewife. It paradigm shift in social freedom woman, helps free herself from dominant sociocultural patterns. draws its power his daring female characters. Actually they are functioned agitate movement l...
Pontormo’s portrait of Alessandro de’ Medici ( c . 1534–35), showing the duke drawing a portrait of his beloved, cleverly engages with the history and fictive nature of art-making. Pliny’s tale about the invention of drawing forms one resonance, Castiglione’s praise of the noble art of drawing another, the theorization of disegno by Vasari and others yet a third. Poetic conventions about the pi...
Life has a curious way of growing us beyond what we can imagine. After 19 years as an emergency physician, I never could have guessed I would become a chaplain. In fact, there were times during those years when I was incapable of comforting a family after giving them the news that a family member had died. I would be so overcome with emotion after 30e60 seconds of the family’s grief, I would sa...
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