a completely randomized design (crd) with three replications was conducted at greenhouse of urmia university in 2013 to study the effect of different methyl jasmonate concentrations (0, 0.1 and 0.5 mm) on the activity of enzymes polyphenol oxidase, phenylalanine ammonia lyase, catalase, ascorbate peroxidase, guaiacol peroxidase and total protein content in basil. activity of the enzymes and tot...

Changes in peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and phenylalanine ammonia-lyase (PAL) activities were studied during Chimonanthus praecox seed maturation. According to our findings the protein content increased steadily from 8 to 12 weeks after flowering, and thereafter decreased significantly. Similarly, SOD and POD activities increased gradually up to 12 weeks after fl...

some possible effects of exogenous application of salicylic acid (sa) and bacillus subtilis on infection potential of root and stem rot disease and on induction of resistance against this pathogen were investigated in laboratory and as well under greenhouse conditions. in vitro, the effectiveness of sa concentrations (2-8mm) on growth of b. subtilis was observed as negative, while sa applicatio...

• Overexpression of MpbHLH in Foc TR4-sensitive Cavendish banana showed that the transgenic lines had superior resistance to Fusarium wilt compared with wild-type (WT). Proteomic analysis phenylalanine ammonia lyase (PAL) and peroxidase (POD) were highly expressed plants. 3. Physiological experiments further revealed plants, especially their roots, high POD activity. Banana ( Musa spp . ) is an...

Biocontrol agents are an environmentally friendly alternative to chemical pesticides against phytopathogens. The effectiveness of metabolites produced by Bacillus atrophaeus B5 for controlling anthracnose Colletotrichum gloeosporioides in harvested fruit and the possible action mechanisms have been studied. Avocado treated with reduced 54% incidence nearly 70% lesion than untreated fruit. treat...

The psoralen compounds, xanthotoxin and 4,5', 8-trimethylpsoralen, when activated, increased phenylalanine ammonia lyase (PAL) activity and the synthesis of pisatin in excised pea pods. Pods presoaked 1 hr with 4,5',8-trimethylpsoralen and then irradiated 4 minutes with 366 nanometer ultraviolet light had twice as much PAL activity 3 hours after irradiation and 12 times as much PAL activity 20 ...

Genetic modification of secondary metabolic pathways to produce desirable natural products is an attractive approach in plant biotechnology. In our study, we attempted to produce a typical soybean isoflavone genistein, a well-known health-promoting metabolite, in non-legume plants via genetic engineering. Both overexpression and antisense suppression strategies were used to manipulate the expre...

Phenylalanine ammonia-lyase, which irreversibly deaminates phenylalanine and tyrosine, markedly inhibited the growth of human leukemic and murine L5178Y lymphoblasts in vitro but had a negligible effect on resting (nondividing) normal lymphocytes. These studies also demonstrated that phenylalanine ammonia-lyase inhibited DNA synthesis in leukemic cells at least as effectively as did asparaginas...

The sequence of a phenylalanine ammonia-lyase (PAL; EC: 4.3.1.24) of the thermophilic and radiotolerant bacterium Rubrobacter xylanophilus (RxPAL) was identified by screening the genomes of bacteria for members of the phenylalanine ammonia-lyase family. A synthetic gene encoding the RxPAL protein was cloned and overexpressed in Escherichia coli TOP 10 in a soluble form with an N-terminal His6-t...