Blood and tear levels of immunoglobulins against herpes simplex virus (HSV) were examined in 28 patients with dendritic keratitis over a period of 28 days. By means of an indirect micro-immunofluorescent technique blood and tear HSV IgG were detected, but neither circulating HSV IgM nor local HSV IgA were found. Over a four-week interval non-diagnostic fluctuations of HSV IgG occurred in most p...

abstract background and aims: herpes simplex virus (hsv) is a common cause of corneal disease and is the leading infectious cause of corneal blindness among developed nations. this study is aimed to provide an estimation of the incidence of herpes simplex virustype-1, 2(hsv1, 2) and varicella zoster virus (vzv) in tears and swab from eye infection by polymerase chain reaction (pcr) in eye disea...

Topical therapy with trypsin, chymotrypsin, and monoxychloroserene, and corneal scrub with iodine, ether, and silver nitrate did not demonstrably reduce the incidence of a positive virus culture from rabbit corneas infected with herpes simplex. It is suggested that the virucidal properties of such substances as measured by their effect on cell-free virus suspensions is not pertinent to the abol...

DURING the early stage of our experiments on the use of low temperature for cataract extraction (Krwawicz, 1961, 1963), we had several opportunities of observing the effects of accidentally touching the cornea with the cryoextractor refrigerated to about -79°C. This accident proved to be quite harmless in all cases as no lasting effect on the clarity of the cornea could be seen after the tissue...

Purpose and Methods: Penetrating keratoplasty (PK) in herpes simplex keratitis (HSK) is increasingly performed not only for visual purposes but also to remove viral antigen from the cornea and to reestablish the structural integrity of the eyes. With the improvement of medical management and surgical techniques which lead to a higher survival rate of the graft, PK in HSK still carries a high co...

In vivo corneal replicas were made in 20 cases of patients with superficial dendritic ulcers of the cornea. Histopathological study of the replicas and superficial epithelial cells showed that the dendrites are composed of rounded epithelial cells and variable sized syncytia containing bizarre shaped nuclei. Pseudopodia-like processes containing DNA and some RNA extend from the syncytia into th...

Five out of 430 patients (1.16%) undergoing kidney transplantation developed an atypical clinical picture of herpetic dendritic keratitis within four weeks after surgery. It was manifested by multiple dendrites, located mainly in the corneal periphery or the limbus, developing in relatively uninflamed eyes. The response to acyclovir therapy was prolonged and took at least three weeks. Additiona...

Aims: To determine the indications for penetrating keratoplasty (PK) at the Corneoplastic Unit and Eye Bank, UK, a tertiary referral centre, over a 10 year period. Methods: Records of all patients who underwent PK at our institution between 1990 and 1999 were reviewed retrospectively. Of the 1096 procedures performed in this period, 784 records were available for evaluation (72%). Results: Regr...

AIMS To determine the indications for penetrating keratoplasty (PK) at the Corneoplastic Unit and Eye Bank, UK, a tertiary referral centre, over a 10 year period. METHODS Records of all patients who underwent PK at our institution between 1990 and 1999 were reviewed retrospectively. Of the 1096 procedures performed in this period, 784 records were available for evaluation (72%). RESULTS Reg...

Herpes simplex virus (HSV)-induced ocular disease is occurring in epidemic proportions throughout the world, and is the number one cause of unilateral corneal blindness in all developed countries. We have found, in a mouse model of herpes simplex keratitis (HSK), that products encoded by the Igh-1 locus on chromosome 12 exert a profound influence on the immune/inflammatory response in the corne...