The red blood cell (RBC) is historically the original parent cell of microparticles (MPs). In this overview, we describe the discovery and the early history of red cell-derived microparticles (RMPs) and present an overview of the evolution of RMP. We report the formation, characteristics, effects of RMP and factors which may affect RMP evaluation. The review examines RMP derived from both norma...

Red cell acetylcholinesterase (AChE) and Na, + K ~adenosinetriphosphatase (ATPase) activities, cell 2,3 diphosphoglycerate (2,3 DPG) and adenosinetriphosphate (ATP) content and filterability ratio were studied in two children (with moderate hemolytic anemia and marked spherocytosis) and their parents. Patients’ parents have no medical problem but evidenced discrete spherocytosis on peripheral s...

OBJECTIVE The diagnosis of hereditary red blood cell (RBC) membrane disorders, and in particular hereditary spherocytosis (HS) and Southeast Asian ovalocytosis (SAO), is based on clinical history, RBC morphology, and other conventional tests such as osmotic fragility. However, there are some milder cases of these disorders that are difficult to diagnose. The application of eosin-5'-maleimide (E...

We studied a patient with a severe spherocytic hemolytic anemia without family history of spherocytosis. Analysis of patient's erythrocyte membrane proteins revealed spectrin deficiency and a truncated alpha spectrin protein. We determined that the patient is a compound heterozygote with two mutations in alpha spectrin gene. Mutation in the paternal allele, designated alpha spectrin(PRAGUE), is...

Objectives:Hereditary spherocytosis is a heterogeneous group of disorder that results in the formation of abnormal red blood cells with fragile cell walls causing anaemia, jaundice, splenomegaly and ultimately gall stone formation. Most children have mild disease do not require splenectomy. Splenectomy is reserved for those with severe disease or who develop symptomatic gall stone. Individuals ...