The full-length Metapenaeus ensis neuroparsin (MeNPLP) cDNA was cloned which encodes a shrimp protein homologous to the insect neuroparsin and vertebrate insulin-like growth factor binding protein (IGFBP). MeNPLP cDNA is 1389 bp in length and the longest open reading frame is 303 bp in length. The first 27 aa are predicted to be the signal peptide and aa 28-101 is the mature peptide with an est...

The response of enzymatic (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPX and glutathione reductase, GR) and non-enzymatic responses (glutathione, GSH, oxidized glutathione, GSSG and GSH/GSSG) against acute Cu toxicity (2-90μg/mL for 48h) in different tissues of Lymnaea stagnalis were measured. Incubation conditions for enzymatic activity measurements were optimized for L...

This study evaluates the influence of dietary vitamin A on growth performance, antioxidant capacity, and lipid metabolism Eriocheir sinensis juveniles (3.95 ± 0.12 g) fed different levels. Crabs were with four diets, including normal (7%) devoid (0 IU/kg, NL-VAD), supplement (6000 NL-VAS), high (13%) HL-VAD), HL-VAS) for 8 weeks. The significant interaction between level was found in weight gai...

We examined the histopathological and hematological response of the Caribbean spiny lobster to experimentally induced infections with Panulirus argus Virus 1 (PaV1). The fixed phagocytes in the hepatopancreas were the primary sites of PaV1 infection in spiny lobsters. Fixed phagocytes were activated in early infections. However, as the disease progressed, the fixed phagocytes became infected an...

2,4-dichlorophenoxyacetic acid and monosodium methanearsonate are often sold in commercial mixtures. Bioconcentration studies have been performed for each of these herbicides individually, but little information exists concerning long-term exposure to a mixture of these herbicides. The following study examined the uptake of arsenic in crawfish after long-term exposure to this mixture, and the h...

Ammonia stress can inhibit the survival and growth, and even cause mortality of shrimp. In this study, ammonia-metabolizing enzyme activities and gene expression were compared between two strains of L. vannamei under different ammonia-N ([Formula: see text]) concentrations (3.4, 13.8, and 24.6 mg/L). The results showed that elevated ammonia concentrations mainly increased glutamine synthetase (...

In order to study the biosynthesis, composition, and origin of sterols in the Florida land crabs, Cardisoma guanhumi (Latreille), we fed 17 male crabs either a cholesterol-free or a high cholesterol diet for 2 to 7 weeks. The origin of sterols in these crabs, whether from biosynthesis or from the diet, was determined by tahree procedures: the incorporation of isotopic mevalonate into the choles...

Freshwater prawns, Macrobrachium lamarrei (Crustacea-Decapoda) were subjected to static subacute exposure (0.755 mg/l) of detergent, Linear Alkyl Benzene Sulphonate (LAS) (25% 96h LC50) for 10,20 & 30 days. Hepatopancreas showed pronounced changes like vacuolization, hypertrophy, hyperplasia and degenerative leading almost complete loss histoarchitecture after days exposure. Peculiar cell shedd...

The Pacific white shrimp Litopenaeus vannamei is an economically important penaeid species worldwide. Under farming conditions, the full ovarian maturation of L. generally depends on combination artificial ablation unilateral eyestalk and feeding high-quality natural diets, suggesting that nutrient accumulation to maturation. In this study, we aimed investigate gene expression related nutrition...