AIMS Recent studies suggest that Helicobacter pylori is an invasive enteropathogen. However, the efficiency with which this pathogen invades mammalian cells remains unknown. Therefore, this study was designed to investigate the invasion frequencies of HEp-2 cells by clinical strains of H pylori. METHODS An acridine orange assay and cultured HEp-2 cell monolayers were used to determine the HEp...

Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D-hep cells, Hepa1-6 cells treated with DMSO for 7 days, could ...

In patients harboring the IR1152 mutant insulin receptor, hepatic glucose production was normally suppressed by insulin. Hepatocytes without the insulin receptor gene and expressing IR1152 (Hep(MUT)) also showed normal insulin suppression of glucose production and full insulin response of glycogen synthase. In contrast, expression of the IR1152 mutant in skeletal muscle maximally increased gluc...

In the framework of Regge phenomenology, masses of the charmed states cq̄ (q = u, d, s) lying on the 13S1-like trajectories are estimated. The overall agreement between our estimated masses and the recent predictions given by modified quark models[hep-ph/0605019, hep-ph/0608011, hep-ph/0608139] is good. Masses of the observed charmed statesDs0(2317), DsJ (2860) and DsJ(2690)/DsJ(2700) can be rea...

The equations of motion of the massless sector of the two dimensional string theory, obtained by compactifying the heterotic string theory on an eight dimensional torus, is known to have an affine o(8, 24) symmetry algebra generating an O(8, 24) loop group. In this paper we study how various known discrete Sand T duality symmetries of the theory are embedded in this loop group. This allows us t...

The limited supply of the Lim Benyesh-Melnick antiserum pools for the typing of enteroviruses has made this test inappropriate for routine use in most clinical laboratories. We studied the correlation between the enterovirus groups and the cell lines on which they displayed cytopathic effect in order to make identifications without using the neutralization test. This study indicated that a pres...

CXCR4 has been reported in various types of human cancer, which is associated with cancer progression and metastasis. However, the investigation of CXCR4 in laryngeal cancer is extremely rare. In the present study, we used lentivirus-mediated shRNA targeting CXCR4 to silenced CXCR4 expression in Hep-2 cells and evaluated the effect of long-term suppression of CXCR4 on Hep-2 growth and metastasi...

Automatic classification of Human Epithelial Type-2 (HEp2) cells staining patterns is an important and yet a challenging problem. Although both shallow and deep methods have been applied, the study of deep convolutional networks (CNNs) on this topic is shallow to date, thus failed to claim its top position for this problem. In this paper, we propose a novel study of using CNNs for HEp-2 cells c...

A comparison has been made of the efficiency of 3 cell systems, human embryo kidney (HEK), Hep 2, and a continuous line of human embryo intestine (HEI), for the isolation of ophthalmic viruses. A total of 40 herpes simplex, 51 adenoviruses, and 2 vaccinia viruses were isolated from 323 specimens. HEK cells were found to be the optimal system, 85 out of 93 (91%) of the viruses being detected in ...

BACKGROUND For detection of anti-nuclear antibodies (ANAs) and antibodies to extractable nuclear antigens (ENAs), samples frequently are screened with indirect immunofluorescence (IIF); further determination of anti-ENA antibodies is performed only when the result is positive. However, because anti-ENA reactivities are found in samples with low fluorescence intensities, we determined anti-ENA a...