We isolated a mutant strain unable to acquire heat shock resistance in stationary phase. Two mutations contributed to this phenotype. One mutation was at the TPS2 locus, which encodes trehalose-6-phosphate phosphatase. The mutant fails to make trehalose and accumulates trehalose-6-phosphate. The other mutation was at the HSP104 locus. Gene disruptions showed that tps2 and hsp104 null mutants ea...

Heat induces an increase in the fraction of cells staining with the nontoxic fluorescent membrane dye dansyl lysine (DL). The fraction of cells excluding DL can, under certain circumstances, be closely correlated to the fraction of cells surviving a heat treatment. Dansyl lysine has previously been shown to select for cholesterol-free membrane domains. We now describe the use of DL to provide r...

The design and response of ground source heat pumps coupled to vertical closed loop arrays in UK domestic applications are investigated in this article. Two typical UK house types are selected as the vehicle for the study and a detailed dynamic thermal modelling method is used to arrive at time-series heating demands for the two houses. A new empirical heat pump model is derived using experimen...

Climate change will worsen the high levels of urban vulnerability in Latin American cities due to specific environmental stressors. Some impacts of climate change, such as high temperatures in urban environments, have not yet been addressed through adaptation strategies, which are based on poorly supported data. These impacts remain outside the scope of urban planning. New spatially explicit ap...

There are a number of errors in the axes labels for Fig 8, “Heat maps for comparative performance analysis of different decomposition levels of wavelet analysis (from left to right: Jaccard index, sensitivity, and specificity).” The publisher apologizes for the errors. Please see the corrected Fig 8 here. There is an error in the axis label for Fig 9, “Heat maps obtained by different methods wi...

The aim of this study was to ascertain whether LY294002, an inhibitor of PI-3K, enhances heat sensitivity in human cancer cells regardless of their p53 status. Colony formation assays showed that LY294002 enhanced heat sensitivity in two human lung cancer cell lines; H1299/wild-type p53 (wtp53) and H1299/mutated p53 (mp53) cells. These cell lines have identical genetic backgrounds except for th...

The transient receptor potential vanilloid 1 (TRPV1) channel is the principal detector of noxious heat in the peripheral nervous system. TRPV1 is expressed in many nociceptors and is involved in heat-induced hyperalgesia and thermoregulation. The precise mechanism or mechanisms mediating the thermal sensitivity of TRPV1 are unknown. Here, we have shown that the oxidized linoleic acid metabolite...

BACKGROUND Environmental changes, air pollution and ozone depletion are increasing oxidative stress, and global warming threatens health by heat stress. We now face a high risk of simultaneous exposure to heat and oxidative stress. However, there have been few studies investigating their combined adverse effects on cell viability. PRINCIPAL FINDINGS Pretreatment of hydrogen peroxide (H(2)O(2)...

The current status and needs of thermal biology and physiol ogy as related to clinical hyperthermia are summarized. Empha sis is placed on heat-induced modification of blood flow and microenvironment in tissues, on the biological effects of heat and X-rays, and on the relationship between drug resistance, heat resistance, and thermotolerance in thermochemotherapy. Re sults from recent studies i...