Plant derived chemicals are valuable sources for a variety of pharmaceuticals, flavors, dyes, fragrance, stimulants, poisons. Hairy roots are induced in susceptible plants by transformation with Agrobacterium rhizogenes. Here we summarize the recent work using a novel bioreactor, the mist reactor, for the culture of hairy roots from many plant species. This study is based on the development of ...

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots T1 Transgenic Plants

Rosmarinic acid is one of the main active components of Coleus blumei and is known to have numerous health benefits. The pharmacological significance of rosmarinic acid and its production through in vitro culture has been the subject of numerous studies. Here, the ability of different tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. ...

The soybean aphid (Aphis glycines) is one of the main insect pests of soybean (Glycine max) worldwide. Genomics approaches have provided important data on transcriptome changes, both in the insect and in the plant, in response to the plant-aphid interaction. However, the difficulties to transform soybean and to rear soybean aphid on artificial media have hindered our ability to systematically t...

Hairy roots are genetically transformed roots and highly recommended sources for various secondary metabolites products. Artimisinin is one of them and frequently utilized as pharmaceutical drug for malaria impairment. Hairy roots are fragile tissue and very much sensitive in regarding to culture inside the bioreactor. Designing of bioreactor and optimising the acquired physical and chemical pa...

Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to improve silymarin pro...

Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantl...

Growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a Salvia sclarea hairy root culture are described. The roots transformed by Agrobacterium rhizogenes (LBA 9402) were cultured in half-strength B5 liquid medium supplemented with 30 g L(-1) sucrose under light (16 h/8 h light/dark). A culture period of 30 days was optimal ...

Withania somnifera (L) Dunal. and Solanum surattense Burm f., the two medicinally important members of family Solanaceae, were investigated for induction of hairy roots using soil borne bacterium, Agrobacterium rhizogenes. Explants like stem segments, hypocotyls and leaves with midrib were infected with the bacterium in vitro. In both plants, extensive hairy roots were induced from leaf explant...

Influence of different Agrobacterium rhizogenes strains on hairy roots induction and secondary metabolites production in Datura innoxia Mill.