Correspondence and reprint requests should be addressed to: Prof. Alejandro Cravioto, Deputy Executive Director ICDDR,B: Centre for Health and Population Research GPO Box 128, Dhaka 1000 (Mohakhali, Dhaka 1212) Bangladesh Email: [email protected] The production of a heat-labile protein unrelated to cholera toxin was described in the 1960s in studies of Escherichia coli isolated from animals ...

Nowadays acute gastroenteritis infection caused by Escherichia coli (E. coli) O157:H7 is frequently associated with hemolytic uremic syndrome (HUS), which usually developed after prodromal diarrhea that is often bloody. The abdominal pain accompanied by failure kidney is a suspicious symptom to develop this disorder. Their pathological characteristic is vascular damage which manifested as arter...

Since the early 1980s, verotoxigenic Escherichia coli serotype O157:H7 has emerged as a major cause of both sporadic and epidemic disease in North America and other regions of the world. Most infections result in mild diarrhea but more serious illnesses include hemorrhagic colitis, hemolytic uremic syndrome, and thrombotic thrombocytopenic purpura. Outbreaks of E. coli O157:H7 infection have oc...

In the present work we developed a MPN quantitative real-time PCR (MPN-qPCR) method for a fast and reliable detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed vegetables. In order to validate the proposed technique, the results were compared with conventional MPN followed by phenotypic and biochemical assays methods. When L. monocytogenes ...

Two Escherichia coli O-rough:K1:H7 strains producing verotoxin 1 that were isolated from stool samples of two travelers with diarrhea who consulted our clinic after trips to the Indian Subcontinent and Central America were characterized. Both strains were sorbitol negative, the same phenotype presented by E. coli O157:H7, but in contrast they were beta-glucuronidase positive. Low-frequency rest...

Shiga toxin-producing Escherichia coli (STEC) are able to cause serious illnesses ranging from diarrhea to hemorrhagic colitis and hemolytic-uremic syndrome (HUS). These bacteria colonize the digestive tract of humans and produce Shiga-toxins, which are considered to be essential for virulence and are crucial in lethal infection. Colon colonization is supposed to be a determinant step in the de...

We investigated two outbreaks of an unusual gastrointestinal illness that affected at least 47 people in Oregon and Michigan in February through March and May through June 1982. The illness was characterized by severe crampy abdominal pain, initially watery diarrhea followed by grossly bloody diarrhea, and little or no fever. It was associated with eating at restaurants belonging to the same fa...

Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology laboratories. With the bias toward the identification of the O157 serogroup in routine diagnostic processes,...

isolate of serotype O123:H– is recorded in the database of the World Health Organization Collaborating Centre for Reference and Research on Escherichia and Klebsiella (F. Scheutz, pers. comm.). Two strains of STEC O123:H– stx2d were isolated from asymptomatic persons in Germany during 1996– 2000 (8). A study in Australia in 2003 reported using a strain of O123:H– stx1 stx2 ehxA from Switzerland...

Avian influenza is a zoonotic disease that can adversely affect humans and animals. Nigeria first reported an outbreak of avian which was caused by subtype H5N1 in 2006, thereafter virological serological surveys revealed the importance local birds live bird markets community at large epidemiology country. In present study, 276 serum samples were collected for testing over five months from appa...