The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated. We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H2O2 at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h. C...

Typical 2-Cys peroxiredoxins (Prxs) react rapidly with H2O2 to form a sulfenic acid, which then condenses with the resolving cysteine of the adjacent Prx in the homodimer or reacts with another H2O2 to become hyperoxidized. Hyperoxidation inactivates the Prx and is implicated in cell signaling. Prxs vary in susceptibility to hyperoxidation. We determined rate constants for disulfide formation a...

Suspension-cultured cells of parsley (Petroselinum crispum L.) were used to study the regulation of extracellular H2O2. After resuspension, the washed cells regulated the H2O2 concentration spontaneously to a constant level that was greatly increased when the cultures were pretreated for 1 d with salicylic acid (SA). The H2O2 level was further increased on addition of a fungal elicitor preparat...

In the absence of external electron donors, oxidized bovine cytochrome c oxidase (CcO) exhibits the ability to decompose excess H2O2. Depending on the concentration of peroxide, two mechanisms of degradation were identified. At submillimolar peroxide concentrations, decomposition proceeds with virtually no production of superoxide and oxygen. In contrast, in the millimolar H2O2 concentration ra...

A luminol chemiluminescence method was used to evaluate the porphyrin-induced photogeneration of hydrogen peroxide (H2O2). This method enabled us to detect H202 in the presence of a high concentration of porphyrin, which was not possible using conventional colorimetry. The limit of detection was about 1 microM. We compared the ability to generate H2O2, using uroporphyrin (UP), hexacarboxylporph...

Background & Aims of the Study: Phenolic compounds have been extensively used in industries for applications such as petrochemical, oil refineries, papers, plastics, steel, pharmaceuticals, textiles, coal conversion, and so on. Specified amounts of Phenolic compounds are lost in the process of their manufacturing and utilization and often cause environmental pollution problems....

Decomposition of hydrogen peroxide (H2O2 ) at physiological levels was studied in human erythrocytes by means of a recently developed sensitive H2O2 assay. The exponential decay of H2O2 in the presence of purified erythrocyte catalase was followed down to 10(-9) mol/L H2O2 at pH 7.4. H2O2 decomposition by purified erythrocyte glutathione peroxidase (GPO) could be directly observed down to 10(-7...

Mitochondria are often regarded as a major source of reactive oxygen species (ROS) in animal cells, with H2O2 being the predominant ROS released from mitochondria; however, it has been recently demonstrated that energized brain mitochondria may act as stabilizers of H2O2 concentration (Starkov et al. [1]) based on the balance between production and the consumption of H2O2, the later of which is...

An efficient and convenient method for degradation of Acid orange 3 (AO3) dye from aqueous solution by combination of Fly ash/H2O2 and ultrasound irradiation is reported. The effects of the relevant operating conditions (pH value, dye concentration, H2O2 concentration, dosage of Fly ash and different reaction system) on the degradation of AO3 were also observed. The combination method was found...

Platinum group metals (PGMs) are important for the manufacture of advanced materials in field catalysts and electronic devices. Since chemical properties PGMs very similar to each other, hydrometallurgical processes should be employed recover with high purity from either ores or secondary resources. In PGMs, first step is dissolution PGMs. For this purpose, inorganic acid solutions oxidizing ag...