Human growth hormone (hGH) gene deletion (6.7 to 7.6 kb) is one of the causes of isolated growth hormone deficiency (IGHD), named IGHD IA. IGHD IA, however, only accounts for about 10% of the total IGHD patients. Most IGHD is caused by unknown mechanisms. Here, hGH gene 5' sequences in three IGHD patients without hGH gene deletion were analysed to see if there was any mutation hindering the exp...

This study was aimed of determining the relationship polymorphism growth hormone receptor gene with some traits (daily rate, relative and specific growth, ratio efficiency feed conversion) in 45 samples common carp (Cyprinus carpio). The following are most important results obtained: DNA sequencing single nucleotide (SNP) showed there three mutations sites C24074T, A24375G G24485A. No genetic m...

A fusion gene containing 5' rat prolactin genomic sequences ligated to the structural portion of the rat growth hormone gene ( grl ) was introduced by DNA-mediated gene transfer into mammalian cells by using a chimeric plasmid vector. Clonal transfected cell lines produced a mRNA that used the authentic 5' initiation site and that was processed to the predicted size. The intracellular levels of...

The regulation of gastrin gene transcription was studied in GH4 pituitary cells transfected with constructs comprised of the first exon of the human gastrin gene and various lengths of 5' regulatory sequences ligated upstream of the reporter gene chloramphenicol acetyltransferase. Gastrin reporter gene activity in GH4 cells was equal to the activity of a reporter gene transcribed from the endog...

In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (...