Abstract In this study, an accurate, simple, economical and precise Reversed-Phase High Pressure Liquid Chromatography (RP-HPLC) method was developed for the simultaneous estimation of Ozenoxacin Benzoic Acid in a pharmaceutical cream formulation, according to International Conference on Harmonisation (ICH) guidelines. Chromatographic separation achieved by gradient elution, RP-HPLC Instrument,...

A method for determination of juglone in leaves and hulls of Pterocarya fraxinifolia was developed, based on RP-HPLC. This compound was extracted from leaves and hulls by chloroform refluxing about 2 hours. The chloroform was evaporated under low pressure at   50 ºC to produce a dry residue. The remained materials were dissolved in acetonitrile to be used for determination of this compound....

BACKGROUND Cod muscle has a balanced protein profile that contains potentially bioactive amino acid sequences. However, there is limited information on release of these peptides from the parent proteins and their ability to modulate mammalian blood pressure. OBJECTIVE The aim of this study was to generate cod antihypertensive peptides with potent in vitro inhibitory effects against angiotensi...

Lutein is one of the major carotenoids in green leafy vegetables (GLVs), which show a marked antioxidant activity. The study was carried out to identify and quantify the carotenoid content of selected Sri Lankan GLVs. RP-HPLC with photodiode array detection method was followed for the separation, identification and quantification of carotenoids. Among the vegetables analyzed, Ipomoea batatas le...

We report a method for the purification of plastoquinone-9 (PQ), a prenylquinone cofactor involved in the photosynthetic electron transport chain. The described procedures relies on spinach-chloroplast isolation followed by PQ extraction and chromatographic fractionation. Extraction of PQ was achieved using partition of chloroplast suspension with methanol:petroleum ether. This procedure remove...

Superoxide anion (O2-.) production from human neutrophils stimulated by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP, 1 microM) was inhibited by preparations of the inhibitor of cAMP-dependent protein kinase, Rp-cyclic adenosine 3',5'-phosphorothioate (Rp-cAMPS, 100 microM). This effect of Rp-cAMPS was reversed by xanthine amine congener (0.1 microM), an adenosine receptor antagonist, an...