Two bacterial strains Lactobacillus fructivorans (AS17B), isolated from adult seabream (Sparus aurata L.) gut, and Lactobacillus plantarum (906), isolated from human faeces, were administered contemporaneously during seabream development using Brachionus plicatilis and/or Artemia salina and dry feed as vectors. Experimental group A received the probiotic strains already via rotifers from day 5 ...

Granulopoiesis is tightly regulated to meet host demands during both " steady-state " and " emergency " situations, such as infections. The transcription factor CCAAT/enhancer binding protein b (C/EBPb) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPb is required are unknown. In this study, a novel flow cytometric method was developed that s...

Peripheral blood granulocytes from patients with chronic myelogenous leukemia (CML) were studied for accessibility of membrane sialic acid and galactose residues to sodium borohydride-3H radiolabeling after oxidation with sodium metaperiodate (PI/B3H4) or with galactose oxidase (GO/B3H4). Granulocytes from untreated patients with chronic myelogenous leukemia showed increased radiolabeling with ...

Human granulocytes clearly play an important role in host defence against invading organisms, however they have also been implicated in the pathogenesis and progression of many chronic inflammatory diseases. In addition, these cells have been paramount in gaining a better understanding of many key-signalling pathways regulating fundamental biological processes. Since granulocytes are terminally...

I N STUDYING certain properties of peripheral leukocytic cathepsins in health and disease,1’2 we found that whole fresh cells contained two pH optima, 3.5 and 8.5, when denatured hemoglobin was used as a substrate.3 Mounter and Atiyeh,4 employing casein as a substrate for extracts of leukocytes, noted optima at pH 3.0, 5.5, and 8.0. Haschen and Krug,5’#{176} using denatured hemoglobin as substr...

The need for sophisticated component therapy has resulted in improved techniques for obtaining concentrates of platelets and granulocytes. The use of single donors as a source for these products is advisable to avoid multiple sensitizations. A method for obtaining large quantities of platelets has been established rather easily. Obtaining concentrated granulocytes represents a greater problem o...

“In-labeled granulocytes were used to study the effects of histocompatibility factors on the migration of transfused granulocytes to infected sites. Fourteen alloimmunized and 20 nonalloimmunized patients received approximately 108 “In-labeled granulocytes from ABO-compatible. non-HIAmatched donors. and scans were performed over known infected sites. All 14 alloimmunized patients had lymphocyto...

We have recently identified a novel population of activated low-density granulocytes (LDGs) in peripheral blood mononuclear cells of HIV seropositive patients. LDGs have a similar morphology to normal density granulocytes (NDGs), but are phenotypically different. Here we measured the expression levels of different phenotypic markers of granulocytes in the blood of HIV seropositive patients at d...

A sensitive confocal Raman microspectrometer was employed to record spectra of nuclei and cytoplasmic regions of single living human granulocytes. Conditions were used that ensured cell viability and reproducibility of the spectra. Identical spectra were obtained from the nuclei of neutrophilic, eosinophilic, and basophilic granulocytes, which yield information about DNA and protein secondary s...

Two studies recently published in Blood have used the pattern of X-chromosome inactivation to test whether white blood cells of patients with paroxysmal nocturnal hemoglobinuria (PNH) and aplastic anemia (AA) belong to a single The investigators’ conclusions were in keeping with data previously obtained by the same approach on red blood cells? However, we find the interpretation of their findin...