OBJECTIVES To identify the gram-negative rods grown from blood cultures and a right-knee fluid aspirate from an 80-year-old caucasian man who had undergone a total right knee arthroplastic procedure 6 years ago, and to assess the genetic similarity between the 2 isolates. METHODS We used 3 different approaches: biochemical testing, matrix-assisted laser desorption ionization time-of-flight (M...

To cite: Kamata K, Tokuda Y. BMJ Case Rep Published online: [please include Day Month Year] doi:10.1136/bcr-2013202876 DESCRIPTION Microscopic stool examination by Gram stain is useful for rapid diagnosis and treatment of Campylobacter infection in patients with acute onset diarrhoea. A 69-year-old Japanese woman with pernicious anaemia presented with a 3-day history of fever and watery diarrho...

During a 6-month study we critically evaluated the accuracy of the AutoMicrobic system Gram-Negative Identification Card (Vitek Systems, Inc., Hazelwood, Mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. Of 356 isolates included in the AutoMicrobic system profile, a total of 307 (86.2%) were correct...

Bacterial vaginosis (BV) is a highly prevalent condition associated with adverse health outcomes. Gram stain analysis of vaginal fluid is the standard for confirming the diagnosis of BV, wherein abundances of key bacterial morphotypes are assessed. These Lactobacillus, Gardnerella, Bacteroides, and Mobiluncus morphotypes were originally linked to particular bacterial species through cultivation...

conclusions flushing dental unit waterlines are an effective measure in reducing the number of bacterial contaminants. results bacterial contamination was found in all 24 units at the beginning of daily activity with a mean colony count of 17850. the number of colonies decreased to 3250 following 30 seconds of flushing in 16 units and later to 1837 after flushing for two minutes in 13 units. in...

aims: the internal unit and icu of hospitals are a suitable environment for growing of bacteria such as staphylococcus epidermidis, pseudomonas and acinetobacters. the aim of this study was to evaluate the efficacy of using disinfectants on bacterial remove in isfahan city, iran, hospitals. instrument & methods: this descriptive study was performed in isfahan city, iran, hospitals in 2015. 200 ...

aims: the internal unit and icu of hospitals are a suitable environment for growing of bacteria such as staphylococcus epidermidis and pseudomonas, acinetobacter. the aim of our study was to evaluate the effect of different disinfectants on bacteria isolated from the surface of hospitals in isfahan. instrument & methods: in this study, 200 samples were randomly selected from different parts of ...

Halogen and aminoalkyl derivatives of methyl 5-methoxy-2-methyl-1-benzofuran-3-carboxylate were prepared using 5-hydroxy-2-methyl-3-benzofuranocarboxylic acid as starting material. (1)H-NMR spectra were obtained for all of the synthesized structures, and for compounds 1 and 2 X-ray crystal structures were obtained too. All derivatives were tested for antimicrobial activity against a selection o...

The bactericidal activity of the novel beta-defensin hBD-3 against 28 species and 55 strains of gram-positive cocci and gram-negative fermentative and nonfermentative rods was tested. All strains proved to be highly or intermediately susceptible to hBD-3 (minimal bactericidal concentration [MBC], 1...