The genetic benefits individuals receive from mate choice have been the focus of numerous studies, with several showing support for both intrinsic genetic benefits and compatibility effects on fertilization success and offspring viability. However, the robustness of these effects have rarely been tested across an ecologically relevant environmental gradient. In particular, sperm environment is ...

Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as densi...

OBJECTIVE To examine the relationship among sperm apoptosis, sperm chromatin status, and DNA ploidy in different sperm fractions. DESIGN Prospective study. SETTING Reproductive research center in a tertiary care hospital. INTERVENTION(S) Sperm prepared by density gradient were evaluated for sperm count, motility, apoptosis, and sperm chromatin assessment. MAIN OUTCOME MEASURE(S) Sperm c...

To evaluate the best method for sperm separation in high DNA fragmentation index (DFI) samples. A prospective randomised study was conducted from 1st January 2019 to 2021. Three hundred patients were by computer generated list and divided into 3 groups. Group (n=100) , which processed microfluidic sorter (MFSS) while group B (n=100), selected Physiological Intracytoplasmic Sperm Injection (PICS...

Sperm exhibit extraordinary morphological divergence yet precise evolutionary causes often remain elusive. A quantitative genetic study sheds light on the major role postcopulatory sexual selection could play in determining sperm size.

Sperm with reduced motility has reduced fertilizing capacity. If low-motile Duroc sperm is mixed with high-motile sperm, the fertilizing capacity of that dose is equal to semen doses exclusively containing high-motile Duroc sperm. With doses that only contained low-motile sperm, the number of total born piglets per litter dropped by minimum 0.5. Duroc boars were selected on the basis of the per...

Sperm require a sense of direction to locate the egg for fertilization. They follow gradients of chemical and physical cues provided by the egg or the oviduct. However, the principles underlying three-dimensional (3D) navigation in chemical landscapes are unknown. Here using holographic microscopy and optochemical techniques, we track sea urchin sperm navigating in 3D chemoattractant gradients....

The aim of this study was to determine the extent of DNA fragmentation and the presence of denatured single-strand or normal double-strand DNA in spermatozoa with extruded nuclear chromatin (ENC) selected by high magnification. Fresh semen samples from 55 patients were prepared by discontinuous isolate concentration gradient. Spermatozoa with normal nucleus (NN) and ENC were selected at 8400× m...

Experiments were conducted to determine the efficiency with which viable, morphologically normal bovine spermatozoa could be isolated using a discontinuous bovine serum albumin (BSA) gradient. In the first experiment, extended semen was layered on top of a BSA gradient (4% BSA over 10% BSA) contained in a 500-ml separatory funnel. When comparing 1, 3, 5, 7, 14 or 21 X 10(9) spermatozoa applied ...