Background: There are some methods for morphology staining of sperm. Although many different and also new methods are used in semen analysis, light microscopy is still used for routine morphological evaluation. The most used of the methods is Papanicolaou staining. This method is routinely used in Andrology laboratory for Human sperm morphology staining. In some domestic animals such as bull, R...

AIM This study quantified the effects of season and photoperiodic treatment on semen and seminal plasma (SP) characteristics in 12 bucks of two Spanish goat breeds (Murciano-Granadina, and Payoya) for the past 1 year. MATERIALS AND METHODS A total of 6 bucks (three of each breed) were exposed to the natural day length and the other six males (three of each breed) were exposed to alternating c...

The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM...

Air tebu dapat dimanfaatkan sebagai pengencer semen karena mengandung berbagai nutrien yang menunjang kehidupan spermatozoa selama preservasi. Penelitian ini bertujuan untuk menguji kemampuan air dalam mempertahankan daya hidup kambing PE preservasi pada suhu 3-5oC. Semen ditampung menggunakan vagina buatan dan segera dievaluasi secara makroskopis mikroskopis. segar dibagi ke lima buah tabung r...

Background: Short time preservation of semen is common in animals (e.g. horse or pig etc…) which their sperm in known as freezing sensitive sperm. It also may use for short transportation of other species to be cryopreserved or use in AI or IVF programs. The aim of the present study was to evaluate effects of duration of cool storage on goat epididymal sperm. Materials and Methods: Total number...