Tumor cells expressing antisense glutaminase RNA show a drastic inhibition of glutaminase activity and they acquire a more differentiated phenotype. We have studied the expression of Sp1 and Sp3 transcription factors in both Ehrlich tumor cells and their derivative 0.28AS-2 antisense glutaminase expressing cells. The expression of phosphorylated Sp1 in 0.28AS-2 cells was 3-fold the expression i...

The provision of glutamine in vivo has been observed to reduce to normal levels the neutrophilia observed after exhaustive exercise and to decrease the neutrophil chemoattractant, interleukin-8. Thus, the role for glutamine in the regulation of inflammatory mediators of human neutrophil activation was investigated. The study sought to establish whether glutamine supplementation in vitro affects...

Glutaminase inhibition enhances the sensitivity of Pten-positive T-ALL to anti-NOTCH1 therapy.

The fact that cancer cells rewire their metabolism to meet their extreme energetic and biosynthetic needs has long been known, since the seminal studies by Otto Warburg demonstrated that cancer cells heavily rely on glycolysis even in the presence of oxygen, the so-called Warburg effect. However, efforts to therapeutically target the special requirements of cancer cells have only gained tractio...

The liver shows net glutamine uptake after a protein-containing meal, during uncontrolled diabetes, sepsis and short-term starvation, but changes to net release during long-term starvation and metabolic acidosis. Some studies report a small net release of glutamate by the liver. The differential expression of glutamine synthetase (perivenous) and glutaminase (periportal) within the liver indica...

the location of extracellular enzyme activities in soil aggregates can be influenced by slope position. this study aims to determine the effect of slope position on aggregate distribution of organic c and l-glutaminase activity. the soils were collected from different slope positions (shoulder, backslope, footslope and toeslope) at two pasture ecosystems (fereydoonshahr and chelgerd, central ir...

Comprehensive genomic and proteomic analyses demonstrate that there is nearly universal activation of the PI3K pathway in glioblastoma (GBM) patients [1]. Persistent PI3K signaling promotes GBM formation and tumor progression in genetic mouse models, establishing PI3K and its effecter mTOR as compelling molecular targets. mTOR, which has two distinct complexes, mTORC1 and mTORC2, is a protein k...