Background Estrogens biosynthesis in ejaculated spermatozoa is an autonomous process that may influence sperm functions. The purpose of this study was to evaluate the relationship between the expression of aromatase, sperm quality and seminal neutral α–glucosidase marker in semen of Tunisian infertile men. MaterialsAndMethods Fifty seven men were investigated: they were composed of two groups: ...

Recently, we reported a novel congenital disorder of glycosylation (CDG-IIb) caused by severe deficiency of the glucosidase I. The enzyme cleaves the alpha1,2-glucose residue from the asparagine-linked Glc(3)-Man(9)-GlcNAc(2) precursor, which is crucial for oligosaccharide maturation. The patient suffering from this disease was compound-heterozygous for two mutations in the glucosidase I gene, ...

the colorado potato beetle, leptinotarsa decemlineata say (col.: chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. in this study, some biochemical aspects of β-glucosidase in the colorado potato beetle were determined. the results showed that β-glucosidase activity in the midgut of adults wa...

Objective Diabetes mellitus is manifested by hyperglycaemia. Different treatments such as diets and drugs are recommended for diabetes control. For various reasons in recent years traditional plant (herbal) therapies as prescribed by indigenous systems of medicine with different mechanisms have commonly been used. The digestive enzymes such as alpha glucosidase are among these herbal remedies....

in this study, accumulation of h2o2, malondialdehyde (mda) (as cold-induced oxidative stress indicators), the transcript levels of dehydrin and beta-glucosidase genes (involved in metabolic responses) was evaluated in chickpea cv. jam, using qrt-pcr during control, cold acclimation (ca), cold stress (cs), recovery, and freezing phases. results showed the existence of wide range of genetic capac...

A strain of bakers' yeast was isolated which could utilize cellobiose and other beta-D-glucosides quantitatively as carbon and energy sources for growth. Cellobiose-grown cells contained a largely cryptic enzyme active against the chromogenic substrate p-nitrophenyl-beta-D-glucoside. The patent (intact cell) activity of such cells was inhibited by azide and, competitively, by cellobiose; neithe...

Gaucher disease type 1, a non-neuronopathic lysosomal storage disease, is caused by mutations at the acid beta-glucosidase locus. Periodic infusions of macrophage-targeted acid beta-glucosidase reverse hepatosplenomegaly, hematologic, and bony findings in many patients. Two patients receiving enzyme therapy developed neutralizing antibodies to acid beta-glucosidase that were associated with a l...

We are investigating the molecular mechanisms involved in the localization of lysosomal enzymes in Dictyostelium discoideum, an organism that lacks any detectable mannose-6-phosphate receptors. The lysosomal enzymes alpha-mannosidase and beta-glucosidase are both initially synthesized as precursor polypeptides that are proteolytically processed to mature forms and deposited in lysosomes. Time c...