Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose-controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built glucose oxidase-polymer hybrids that assemble in presence an organometallic catalyst: hemin. The design hybrid is optimized to increase effectiveness directional channeling at low substrate concentration. Importantly...

determined by six different analytical methods, i.e., the glucose-oxidase method (G), the autoanalyser method with Hoffman's method (A), the Somogyi-Nelson method (S), the o-toluidine method (T), the Momose's method (M) and the Hage dorn-Jensen method (H). The values determined by micromethods of HagedornJensen, of Momose and of Somogyi-Nelson are usually higher, and the values obtained by o-to...

Glucose oxidase is one of the most conspicuous commercial enzymes due to its many different applications in diverse industries such as food, chemical, energy and textile. Among these applications, the most remarkable is the manufacture of glucose biosensors and in particular sensor strips used to measure glucose levels in serum. The generation of ameliorated versions of glucose oxidase is there...

A new sensing approach, employing diffraction gratings of hydrogel functionalized with glucose oxidase (GOx), was developed for quantitative glucose detection.

We report here that cultured human lymphoma cells in the absence of sonicated eosinophils are sensitive to killing by glucose oxldase (@3-Dglucose:oxygen..oxido reductase; EC 1.1.3.4) at concentrations as low as 0.025pg/mI,a levelthat canberapidlyattainedin s.c.tumor implantsin mice that receive a single nonlethal injection of enzyme. Multiple clono genie assays were used to measure the surviva...

We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine d...

The simplest transducer in the development of potentiometric biosensors is the glass pH electrode. Glucose oxidase immobilization was achieved using cellophane [1], nylon [1-6] or nitrocellulose [5,7] membranes that are subsequently fixed on the sensitive bulb of the pH electrode that senses the pH diminution, as a result of the biocatalytical reaction occurring in the enzyme layer (glucose oxi...

An insulin delivery system that self-regulates blood glucose levels has the potential to limit hypoglycemic events and improve glycemic control. Glucose-responsive systems have been developed by coupling oxidase with a stimuli-responsive biomaterial. However, challenge of achieving desirable release kinetics (i.e., within minutes after elevation duration on order weeks) still remains. Here, we ...

BACKGROUND Diabetic cardiomyopathy (DCM) suggests a direct cellular insult to myocardium. Hyperglycemia-induced oxidative stress and apoptosis have been implicated in the pathogenesis of DCM. NADPH oxidase is a major source of reactive oxygen species (ROS) generation in cardiomyocytes. Resveratrol, a naturally occurring polyphenol, has shown beneficial effects on some cardiovascular complicatio...

The glucose oxidase-peroxidase method for determining serum glucose, with the ammonium salt of 2,2’-azine-di (3 ethyl benzothiazoline-(6)-sulfonic acid) as the chromogen, was adapted to use with the single-channel AutoAnalyzer and the SMA 12/60 (Technicon Corp.). The extended linearity (up to 500 mg/100 ml on both systems) eliminated the need for numerous dilutions in the 300 to 500 mg/100 ml r...