Neoplasms result from the uncontrolled clonal proliferation of abnormal or transformed cells. The early stages of this process are difficult to study because of the lack of sensitive and specific markers of clonal evolution in an experimental system. We have developed a cat model using cellular mosaicism for glucose-6-phosphate dehydrogenase (G-6PD). Our findings confirm that the structural loc...

.I T ORKERS IN ITALY,’ Israel”” and Americ&OU have shown that the V V gltmtathione ( GSH ) instability and decreased glucose-6-phosphate dehydrogenase ( C-6-PD ) activity, which are characteristic of primaquine sensitive imldividuals, are also present in persons who develop acute hemolytic anemia following ingestion of fava beans. The above authorsi ,:i, ii .12 have presented evidence that the ...

Human erythrocyte glucose 6-phosphate dehydrogenase (o-glucose 6-phosphate :nicotinamide adenine dinucleotide phosphate oxidoreductase, EC 1.1.1.49) was purified by column chromatography with diethylaminoethyl cellulose, calcium phosphate gel, carboxymethyl cellulose, diethylaminoethyl Sephadex, and carboxymethyl Sephadex. A homogeneous preparation was obtained in over-all yield of about 50%. T...

The direct oxidative pathway of glucose utilization or the hexose monophosphate shunt has received a great deal of attention recently, and numerous attempts have been made to evaluate its importance in various tissues. Glock and McLean, in making a survey of the levels of enzymes of this pathway in a number of mammalian tissues, recently reported unusually high activity in the adrenal glands, p...

Glucose 6-phosphate dehydrogenase from Zeuconostoc mesenteroides, previously isolated in crystalline form (Olive, C., and Levy, H. R., Biochemistry, 6, 730 (1967)), is shown to be essentially homogeneous by disc gel electrophoresis and sedimentation velocity analysis. The weight average molecular weight is 103,700, determined by both high speed and low speed sedimentation equilibrium techniques...

In a Chinese population 1,000 full-term male neonates and a further 117 jaundiced neonates of both sexes were studied in an investigation of the frequency of deficiency of erythrocyte glucose-6-phosphate dehydrogenase (G6PD). This enzyme was found to be deficient in 3.6% of male neonates. Correlation of the results with the birthplace of the 602 mothers who were known to come from Kwangtung pro...

Yeast glucose-6-phosphate dehydrogenase (G6PDH) in solutions exists as the equilibrium mixture of dimers and tetramers. Only dimers are active. The equation responding to these conditions was used to determine the parameters: the specific activity of dimers and the dissociation equilibrium constant of tetramers. The stability of G6PDH dimers were shown to be maintained due to the conformational...

Glucose-6-phosphate dehydrogenase (G6PD) is the key enzyme that initiates pentose phosphate cycle in glucose metabolism. Physiologically, this pathway main source of nicotinamide adenine dinucleotide (NADPH) for red blood cells. This study aims to identify glucose-6-phosphate mutations Nung ethnic patients with G6PD deficiency. 18 pediatric group were diagnosed deficiency at Vietnam National Ch...

Dark activation of light-inactivated glucose-6-phosphate dehydrogenase was inhibited by catalase in a broken pea chloroplast system. Partially purified glucose-6-phosphate dehydrogenase from pea leaf chloroplasts can be inactivated in vitro by dithiothreitol and thioredoxin and reactivated by H(2)O(2). The in vitro activation by H(2)O(2) was not enhanced by horseradish peroxidase, and dark acti...