N-acetyl-B-D-Glucosaminidase was purified by affinity and ionexchange chromatography. Two major, A and B, and three minor intermediate forms were isolated and characterized. NAG-A and NAG-B were purified 440 and 1200 fold with final yields of 16 and 23 percent respectively. Each activity was represented by a single protein band. After 70 min preincubation at 55°C a loss of70% activity of N...

At present, there is no established diagnostic method by which the metastatic ability of an individual prostatic cancer can be accurately predicted. Metastasis is a multistep process, the first critical step of which is invasion. Tumor invasion has been suggested to involve a variety of hydrolytic enzyme activities; therefore, the tumor levels of these activities might be indicative of the over...

Fibroblasts from patients with the Sanfilippo syndrome fall into two subgroups, each manifesting a deficiency of a specific factor required for normal metabolism of sulfated mucopolysaccharide. The factor deficient in the A subgroup has been purified 850-fold from normal human urine by ammonium sulfate precipitation, successive chromatography on Sephadex G-ZOO, carboxymethylcellulose, and hydro...

Several lysosomal enzymes present in human plasma (N-acetyl-beta-glucosaminidase, beta-glucuronidase, beta-galactosidase, alpha-galactosidase, alpha-L-fucosidase, alpha-mannosidase, beta-glucosidase) were maintained in a fully active state for at least 8 months by the addition of ethylene glycol (300 milligrams final concentration) to freshly prepared plasma and storage at -20 degrees C. Pools ...

Microplastic pollution is a topic of increasing concern, especially since this issue was first addressed in soils. Results have so far been variable terms effects, suggesting that there substantial context-dependency microplastic effects soil. To better define conditions may affect microplastic-related impacts, we here examined as function shape and polymer type, tested if on soil properties mi...