background: the mainstay of diagnosis of relapsing fever (rf) is demonstration of the spirochetes in giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. this study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. methods: animal blood sampl...

BACKGROUND The mainstay of diagnosis of relapsing fever (RF) is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by microscopy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spirochetemia. METHODS Animal blood samples w...

The reliability of eight distinct methods (Giemsa staining, trypan blue exclusion, acridine orange/ethidium bromide (AO/EB) double staining for fluorescence microscopy and flow cytometry, propidium iodide (PI) staining, annexin V assay, TUNEL assay and DNA ladder) for detection and quantification of cell death (apoptosis and necrosis) was evaluated and compared. Each of these methods detects di...

AIM To determine whether two recently described staining methods (the modified McMullen's and the Helicobacter pylori silver stain HpSS methods) used for the histological identification of H pylori organisms are superior to two established techniques (the modified Giemsa and anti-H pylori antibody immunostain) in terms of availability, reproducibility, rapidity, sensitivity, and cost. METHODS...

Chromatin organization in the holocentric chromosomes of the aphid Brevicoryne brassicae has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA3 staining. C-banding technique showed the presence of heterochromatic bands on both telomeres of the two X chromosomes, whereas only the longest pair of autosomes show distinct intercalary C-positive bands. Moreover, sil...

OBJECTİVE: In this study, we aimed to examine of antileishmanial effect curcumin-mediated sonodynamic (SDT), photodynamic (PDT), and sonophotodynamic (SPDT) therapies with a potential mechanism against the Leishmania tropica (L.tropica) promastigotes. SPDT is new treatment modality for Leishmaniasis that combines therapies. Curcumin natural antiinflammatory agent has been used treating medical ...

Background: Bovine theileriosis is a tick-born disease in tropical and subtropical regions of the world. The study was aimed to detect Theileria sp. at genus level blood samples both local imported feedlot bulls by direct Giemsa staining, ELISA, nested polymerase chain reaction. Methods: A total 250 (125 125 bulls) were collected randomly. from five different districts Duhok Governorate Kurdist...

Background Circulating tumor cells (CTCs) could escape from the immune system through the programmed death-ligand 1 (PD-L1)/programmed cell death protein 1 (PD-1) axis leading to the development of metastasis. The current study investigated the expression of PD-1/PD-L1 on CTCs isolated from non-small cell lung cancer (NSCLC) patients treated with chemotherapy. Patients and methods CTCs were i...

Australian spiders of the genus Abstract. -Three species of the spider genus Physocyclus (P. calijbrnicus, P. enaulus. P, sp.) . SOC. 96:359-362. were karyotyped using an air-drying, Giemsa staining method. All chromosomes were metacentric, with males being 2n = 15 (N = 7 + XO) and females being 2n = 16. The karyotyping method resulted in slide preparations which were countable five years post-...