Cultures of Azotobacter chroococcum strain A6 were grown for 14 days in a nitrogen-deficient mineral medium, the supernatant fluid and bacteria extracted and examined by paper partition chromatography with two solvent systems which separate authentic gibberellin (GA 3) and indolyl-3-acetic acid (IAA). Gibberellin-like substances were not detected on the chromatograms examined under ultraviolet ...

The 'Chinese lantern' phenotype or inflated calyx syndrome (ICS)--inflated sepals encapsulating the mature berry of Physalis floridana--is a morphological novelty within the Solanaceae. ICS is associated with heterotopic expression of MPF2, which codes for a MADS-box transcription factor otherwise involved in leaf formation and male fertility. In accordance with this finding, the MPF2 promoter ...

Gibberellins (GAs) are involved in the regulation of flowering and fruit-set in grapes (Vitis vinifera L.), but the molecular mechanisms behind this process are mostly unknown. In this work, the family of grapevine GA oxidases involved in the biosynthesis and deactivation of GAs was characterized. Six putative GA 20-oxidase (GA20ox), three GA 3-oxidase (GA3ox), and eight GA 2-oxidase (GA2ox) pr...

Barley endosperm halves release acid phosphatase in response to several gibberellins and gibberellin precursors. Seed halves incubated with 10(-7)m GA(3) at 29 degrees begin to release phosphatase after 11 hr and release it for another 26 hr in response to GA(3). After 37 hr, the rate of release slows to that of seed halves incubated without GA(3). GA(3) is active at 10(-10)m and maximally acti...

Bacteria have evolved gibberellin phytohormone biosynthesis independently of plants and fungi. Through 13C-labeling and NMR analysis, the mechanistically unusual "B" ring contraction catalyzed by a cytochrome P450 (CYP114), which is the committed step in gibberellin biosynthesis, was shown to occur via oxidative extrusion of carbon-7 from ent-kaurenoic acid in bacteria. This is identical to the...

Patatin-related phospholipase A (pPLA) hydrolyses glycerolipids to produce fatty acids and lysoglycerolipids. The Oryza sativa genome has 21 putative pPLAs that are grouped into five subfamilies. Overexpression of OspPLAIIIα resulted in a dwarf phenotype with decreased length of rice stems, roots, leaves, seeds, panicles, and seeds, whereas OspPLAIIIα-knockout plants had longer panicles and see...

Two fltnorene-9-carboxylic acid derivatives (fig 1, I and II) have recently been shown to be potent growth regulators in higher plants (1), and have been givein the trivial name, morphactins. The structtural similarity between the morphactins and gibberellic acid (fig 1, III) immediately suggested a common site of action. In fact, Ziegler, et al. (4) have claimed that methyl-morphactin (I) is a...

(ibl)berellinl-like stulstances occtlr in mianv higher pllants (1 2, 17, 20). and sexeral have beenl idenitified (12). Tlhe presence of gibberelliln-like activity in seedless fruit, of V'itis v'iiifcra L. was (lenlonstrated in 1960 by Coombe (1). Although it seemiis probable that sulch a widely spread hormonie also would be invxolved in the groxwth of seede(d fruit, Coombe was unable to demnons...

Jatropha curcas L. seeds an oilseed plant with great potential for biodiesel production. However, low seed yield, which was limited by its lower female flowers, was a major drawback for its utilization. Our previous study found that the flower number and female-to-male ratio were increased by gibberellin treatment. Here, we compared the transcriptomic profiles of inflorescence meristem at diffe...