Apolipoprotein E (apoE) plays an important role in lipid metabolism. Its epsilon4 allele has been consistently associated with lipoprotein disorders but its connection to myocardial infarction (MI) is controversial. Because epsilon4 frequency decreases with age we thought that the contradictory results in different studies could be due to the wide age range of the subjects included. To test our...

OBJECTIVE The present study aims to investigate the correlation between Interleukin (IL)-17 gene polymorphism with gastric cancer susceptibility in Han Chinese population. PATIENTS AND METHODS Between November 2013 and October 2014, 386 patients with gastric cancer who had undergone surgeries at our institution and 374 age- and sex-matched healthy controls were included in this study. Single ...

Brazil is a country of continental dimension with a population of different ethnic backgrounds. Thus, a wide variation in the frequencies of hepatitis C virus (HCV) genotypes is expected to occur. To address this point, 1,688 sequential samples from chronic HCV patients were analyzed. HCV-RNA was amplified by the RT-PCR from blood samples collected from 1995 to 2000 at different laboratories lo...

The myostatin (MSTN) gene was studied as a candidate genetic marker for growth traits. We investigated polymorphisms of the MSTN gene in 664 individuals from four goat populations and applied PCR-SSCP and DNA sequencing analysis to reveal two single nucleotide polymorphisms (DQ167575: g.368A>C (p.Lys49Thr) and g.4911C>T. At g.368A>C locus, the frequencies of g.368A allele were 0.75–0.81, and th...

A total of 423 blood samples were collected (during 2009 and 2010) from all the ram holdings at three major Jordanian governmental Awassi breeding stations (Al-Khanasry, Al-Mushairfa and Al-Fjaje) and two private flocks. All blood samples were screened for the presence of mutations at the CNGA3 gene (responsible for day blindness in Awassi sheep) using RFLP-PCR. The day blindness mutation was d...

INTRODUCTION ABO blood group system is the most important blood group in transfusion and has been widely used in population studies. Several molecular techniques for ABO allele's detection are widely used for distinguishing various alleles of glycosyl transferase locus on chromosome 9. METHODS 744 randomly selected samples from Azari donors of East Azerbaijan province (Iran) were examined usi...

Associations between polymorphisms of the CD36 gene and susceptibility to coronary artery heart disease (CHD) are not clear. We assessed allele frequencies and genotype distributions of CD36 gene polymorphisms in 112 CHD patients and 129 control patients using semi-quantitative polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Additionally, we detecte...

BACKGROUND The purpose of this study was to evaluate the potential association of five (p.P47S, p.R72P, PIN3 Ins16bp, p.R213R and r.13494g>a) polymorphisms of TP53 with the risk of developing breast cancer in North Indian Punjabi population. METHODS We screened DNA samples of 200 sporadic breast cancer patients (197 females and 3 males) and 200 unrelated healthy, gender and age matched indivi...

polymorphism of the b-lactoglobulin (b-lg) gene in 101 cows belonging to the holstein herd and a superior cow, producing more than 150 kg milk/day, together with four offsprings was investigated by the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method. in the holstein herd, the alleles a and b of the b-lg gene had frequencies of 0.53 and 0.47, respectively. th...

the objective of this study was to determine the polymorphism of bovine β-lactoglobulin (β-lg) gene and its association with milk production traits in iranian najdi cattle. blood samples were collected from 80 najdi cattle from the najdi cattle breeding station located in shoshtar, khuzestan. polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) techniqus were used...