PURPOSE Several barriers that collectively restrict gene delivery by viral vectors in vivo have been described. Previously, we identified soluble chondroitin sulfate-proteoglycans/glycosaminoglycans in malignant pleural effusions (MPEs) as inhibitors of retroviral vector transduction. Soluble components of MPE also inhibited adenoviral (Ad) gene transfer, and the factors were characteristically...

conclusions mg-63 and saos-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts. background a large number of phospholipase c (plc) enzymes, both mrna transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. mg-63 and saos-2 human ost...

An Hfr13 Delta(proA-lac) deletion recipient, -Delta(proA-lac)-F-purE(+)-, has been utilized in a study of the origins of duplications formed during chromosome fragment integration. Among the Pro(-)Lac(+) transductants, some have duplications spanning the F locus. These transductants are, or segregate, strains with F' episomes carrying genes of the duplication. Some of the duplications include p...

African swine fever virus (ASFV) has been identified as the agent of fever, resulting in a mortality rate nearly 100% domestic pigs worldwide. Protein p22 encoded by gene KP177R reported to be localized at inner envelope virus, while function remains unclear. In this study, interacting proteins host were high-throughput method and analyzed Gene ontology terms Kyoto Encyclopedia Genomes (KEGG) p...

We compared the efficiency of transduction by an HIV-1-based lentiviral vector to that by a Moloney murine leukemia virus (MLV) retroviral vector, using stringent in vitro assays of primitive, quiescent human hematopoietic progenitor cells. Each construct contained the enhanced green fluorescent protein (GFP) as a reporter gene. The lentiviral vector, but not the MLV vector, expressed GFP in no...

BACKGROUND The development of a convenient high-throughput gene transduction approach is critical for biological screening. Adeno-associated virus (AAV) vectors are broadly used in gene therapy studies, yet their applications in in vitro high-throughput gene transduction are limited. PRINCIPAL FINDINGS We established an AAV reverse infection (RI)-based method in which cells were transduced by...

PURPOSE The lacrimal gland (LG) delivers defensive and metabolic factors to the ocular surface. These functions may be disrupted in several diseases, and for most of them there is no cure. The aim of this study is to investigate conditions and limitations for using adeno-associated virus (AAV) vectors as gene transfer agents to LG. METHODS Eight-week-old Balb/c mice were used to investigate r...

Adeno-associated virus (AAV) is a promising vector for human gene therapy. Although more effective than non-viral vectors, AAV still requires improvement in efficacy in order to become a successful gene therapy vector. With this in mind, we have sought to identify and examine identified enhancers of adeno-associated virus type 2 (AAV2) transduction. Using a high throughput screening system with...

The clinical application of gene transfer is hindered by the availability of the multipotential stem cells and the difficulty in obtaining efficient retroviral transduction. To assess potential means by which gene transfer into human hemopoietic stem cells might be enhanced, the retroviral transduction efficiency of human bone marrow cells (BM) or peripheral blood progenitor cells (PBPC) was co...

Retroviral vector-mediated gene transfer into endothelial cells is relatively inefficient with transduction rates as low as 1-2% in vitro and even lower in vivo. To increase the efficiency of gene transfer into endothelial cells, we used retroviral vectors expressing beta-galactosidase and urokinase and measured endothelial cell transduction efficiencies with quantitative assays for beta-galact...