Anchote is a tuber crop indigenous to Ethiopia. Starch hydration properties and important gel characteristics which include: color, rheological (at 2, 4, 6, 8, 10% starch:water w/w) texture evolution w/w), during 0 192 h storage 4 °C), of anchote starches isolated from four cultivars (Desta 01, Desta 24, white red) were evaluated compared with potato cassava (PS CS). The lightness (L*) whitenes...

Here, the application of the gel-forming carbohydrate polymer, gellan gum, for the electrophoresis and isolation of DNA is detailed. Gellan gun forms gels in the presence of divalent metal cations, and the gels can be converted back to a solution by the addition of a chelating agent such as EDTA. Also, gellan electrophoresis gels can be formed using diamines. These gels are reversible by increa...

The evaluation of proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis is a common technique used by biochemistry and molecular biology researchers. For laboratories that perform daily analyses of proteins, the cost of commercially available polyacrylamide gels (~$10/gel) can be considerable over time. To mitigate this cost, some researchers prepare their...

Pulsed field gel electrophoresis (PFGE), the gold standard of molecular typing methods, has a major disadvantage of an unusually long electrophoretic time. From the original protocol of 6 days, it was modified to 3 days and subsequently to a single day. We describe the procedure of stacking five to six gels one on top of another in order to increase and maximize the output in a shorter time wit...

ketorolac tromethamine (kt) containing microemulsion-based gels (mbgs) have been formulated, based on the previous phase diagram studies, using a pharmaceutically acceptable surfactant (soybean lecithin; epikuron 200) and oil (isopropyl myristate; ipm) and the effect of formulation variables on the release profile of the drug from mbgs through intact guinea pig skin and various artificial membr...

monolithic aerogels of high molecular weight polyethylene (mw= 3x106- 6x106 g/mol) have been prepared by solvent extraction with supercritical carbon dioxide from thermoreversible gels prepared in decalin. these low density and highly porous aerogels present an apparent porosity up to 90%. the aerogel morphology observed by scanning electron microscopy (sem) is characterized by spherulitic stru...

background: the purpose of this study was to evaluate the dependency of magica polymer gel dosimeter response (r2) on different electron energies as well as on different mean dose rate for a standard clinically used linear accelerator. materials and methods: the sensitivity of the dosimeter was represented by the slope of calibration curve in the linear region measured for each modality. a cali...

background: in this work investigation of the normoxic pagat polymer gel dosimeter such as sensitivity, the r2-dose response with post time and the percentage depth dose (pdd) of pagat polymer gel dosimeter have been undertaken. materials and methods: using mri, the formulation to give the maximum change in the transverse relaxation rate r2 was determined to be 4.5% n,n'-methylenbis- acryl...

Regeneration after surgery can be improved by the administration of anabolic growth factors. However, to locally maintain these factors at the site of regeneration is problematic. The aim of this study was to develop a matrix system containing human mesenchymal stem cells (MSCs) which can be applied to the surgical site and allows the secretion of endogenous healing factors from the cells. Calc...

Double network (DN) gels, consisting of a brittle first and flexible second network, have been known to be extremely tough and functional hydrogels. In a DN gel subjected to force, the brittle first network breaks prior to the fracture of the flexible network. This process, referred to as internal fracture, dissipates energy and increases the energy required to completely fracture DN gels. Such...