An alteration of the blood serum proteins in patients with catarrhal jaundice has been amply established by the development of a positive cephalin flocculation (1). A standard emulsion composed of cephalin and cholesterol, which remains stable in 0.2 ml. to 0.1 ml. normal serum, diluted 1: 21, tends to flocculate and precipitate when added under identical conditions to the serum of patients wit...

The antigenic properties of the polypeptide chains of human 7S gamma-globulin have been related to two major non-cross-reacting antigenic determinants of the whole molecule. These determinants, called S and F, were obtained by hydrolysis of gamma-globulin with papain. Antisera against whole gamma-globulin and against S and F fragments were used in techniques of immune diffusion. Light (L) chain...

The concentration of gamma globulin in the serum of apparently healthy adults from tropical areas is considerably higher than that of residents in temperate zones (Holmes, Stanier, Semambo and Jones, 1951; Satoskar and Lewis, 1954; Deegan, Gilles and McGregor, 1956; Kulkarni, 1957b; Edozien, 1957). In studies of plasma protein patterns in 100 Indian medical students (Satoskar and Lewis, 1954) g...

The serum protein patterns of groups of Nigerians including 98 children partially protected by "daraprim" against malaria, and 113 unprotected children, have been studied. The protected children have a significantly lower concentration of serum gamma globulin than the unprotected children from the age of 12 months onwards. In children, sicklers have a significantly higher serum gamma globulin t...

1. The half-lives of homologous gamma globulins labelled with I(131) agree well with the half-lives of passively administered homologous antibodies and provide a simple, quantitative tool for the determination of antibody half-life when immunochemical techniques are difficult to apply. 2. The half-lives of gamma globulin or antibody vary markedly from species to species and apparently even amon...

The ability of serum factors to cross-link labeled mouse monoclonal antibody (mAb) of irrelevant specificity (mAb FN61, subclass IgG1) to different particle types coated with sheep IgG, bovine gamma-globulin, or mAb FN61 was measured simultaneously by flow cytometry. Significant interference with mAb FN61-coated particles was detected in 53 of 101 sera. Of the 30 sera showing the most pronounce...

Three human serums containing artificially produced blocking antibody against low ragweed allergen were studied for the possibility of relating blocking antibody to electrophoretically definable components. An adaptation of the qualitative passive transfer test to quantitative interpretation is described, methods and procedures are given and uncertainties and possible errors due to lack of prec...

Serological procedures useful for the detection of rheumatoid factors, utilizing polystyrene latex particles, have become well established and show a reasonably good degree of specificity and sensitivity (Mayne and Mathieson, 1961; Plotz and Singer, 1956; Vaughan, 1959). The use of these tests has been reviewed by Singer (1961) and Waller, Decker, Toone, and Irby (1961). The tests have also bee...