The estimation of galactose in plasma by the enzyme system galactose oxidaseperoxidase was investigated. The method was found to be relatively specific for galactose although xylose and ascorbic acid reacted to a slight degree with the enzyme; lactose also reacted with the enzyme, probably because partial hydrolysis of the disaccharide released galactose. The relationship between absorbance and...

I describe a rapid quantitative centrifugal analysis for galactose in blood. The technique involves galactose dehydrogenase. As compared to the manual (o-toluidine) method for galactose determination, analysis time (4 min 45 s on the GEMSAEC, 4 min 10 s on the CentificCHEM) and sample volume (5 microliter) were considerably reduced. Sample deproteinization is eliminated. A modified commercial ...

Topper and Stetten (1) fed n-galactose-l-Cl4 by stomach tube to fasted rats and isolated glucose from the liver glycogen. About 90 per cent of the Cl4 was in C-l of the glucose unit of glycogen. They concluded that a direct epimerization of carbon 4 of the n-galactose occurred rather than a rupture of the carbon skeleton (1). However, any mechanism which removed C-l and which formed glycogen fr...

Clinical evidence suggests an association between galactosaemia and premature ovarian insufficiency (POI); however, the mechanism still remains unresolved. Experimental galactose toxicity in rats produces an array of ovarian dysfunction including ovarian development with deficient follicular reserve and follicular resistance to gonadotrophins that characterize the basic tenets of human POI. The...

Scope A high fructose and galactose intake show adverse metabolic effects in animal models humans, but it is yet unknown if addition of fermentable dietary fiber can mitigate such effects. This study investigate the intakes galactose, with/without added fructooligosaccharides (FOS), on factors, inflammation, gut integrity markers rats. Methods Results Rats (n = 6/group) receive different carboh...

In Lactococcus lactis subsp. cremoris FD1, galactose and lactose are both transported and phosphorylated by phosphotransferase systems. Lactose 6-phosphate (lactose-6P) is hydrolyzed intracellularly to galactose-6P and glucose. Glucose enters glycolysis as glucose-6P, whereas galactose-6P is metabolized via the tagatose-6P pathway and enters glycolysis at the tagatose diphosphate and fructose d...

Paucilactobacillus wasatchensis, a nonstarter lactic acid bacteria, can cause late gas production and splits cracks in aging cheese when it metabolizes 6-carbon substrates, particularly galactose, to 5-carbon sugar, resulting the release of CO2. Previous studies have not explained no galactose is present. Based on genome sequence Pa. wasatchensis WDC04, genes for potential metabolic pathways we...

d-galactose has been considered a senescent model for age-related neurodegenerative disease. It induces oxidative stress which triggers memory impairment, neuroinflammation and neurodegeneration. Caffeine act as anti-oxidant and has been used in various model of neurodegenerative disease. Nevertheless, the effect of caffeine against d-galactose aging murine model of age-related neurodegenerativ...