Rabbit corneas were frozen and thawed by three methods and compared by full thickness transplantation as well as specular microscopy, histology, and transmission electron microscopy. Two of the methods used a recently described technique, in which the excised cornea was immersed in a potassium-rich buffered solution containing the cryoprotectant dimethyl sulphoxide (Me2SO, 2 mol/l). This soluti...

We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. ...

Controlled slow-freezing is the procedure currently applied for immature testicular tissue (ITT) cryobanking in clinical practice. Vitrification has been proposed as a promising alternative, with a view to better preserve the spermatogonial stem cells for future fertility restoration by autografting in young boys suffering from cancer. It appears that besides the potential influence of the cryo...

Freezing action always results in great damage to the pore structure and fractal characteristics of freezing–thawing soft soil. Nuclear magnetic resonance (NMR) can help collect data describe microstructure frozen–thawed This paper conducted a series nuclear tests interpret effect freezing duration, pressure temperature on soil’s micro-pore characteristics. The size distributions (PSDs) tested ...

This study is a long-term evaluation of the total pregnancy potential of cohorts of fresh and cryopreserved sibling embryos from in-vitro fertilization (IVF) cycles stimulated with either the gonadotrophin-releasing hormone analogue buserelin (BUS) (long protocol) or clomiphene citrate (CC) both in combination with human menopausal gonadotrophin (HMG). Therefore a retrospective analysis was per...

To the Editor: The recent introduction of a general coagulation function test, namely the thrombin-generation assay (TGA), has enabled efficient assessment of the global functioning of the hemo-static system. By using a fluorogenic substrate, the TGA produces throm-bin-generation curves in a fully automated manner that may be useful and sensitive enough to screen for either hypercoagulable stat...

The effects of meat shape and size, freezing method and thawing temperature on the drip loss of beef brisket and the protein content of its thaw exudates were examined in this study. The beef brisket was cut into the following shapes: cube; brick-shape with rectangle cross-section; and brick-shape with square cross-section and were then frozen using a standard freezer (-15 oC), an air-blast fre...

In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

Study question: Does the type of medium used to culture fresh and frozen-thawed embryos influence neonatal birthweight after single embryo transfer (SET) in IVF? Summary answer: A comparison of two commercially available culture media showed no significant influence on mean birthweight and mean birthweight adjusted for gestational age, gender and parity (z-scores) of singletons born after a fre...

Abstract. Soil microwave permittivity is a crucial parameter in passive retrieval algorithms but remains challenging variable to measure. To validate and improve satellite data products, precise reliable estimations of the relative (?r=?/?0=??-j???; unitless) soils are required, particularly for frozen soils. In this study, measurements were acquired using two different instruments: newly desig...