BACKGROUND The aim of the study was to evaluate the quality of fresh raccoon dog semen and raccoon dog semen stored at 4 °C. The qualitative evaluation was based on apoptosis in the sperm cells, which was tested by the Annexin V/Pi assay, the TUNEL method and JC-1. In addition, the suitability of the semen for insemination and its effect on reproduction in females were determined in relation to...

The aims of the present study were to determine the number of spermatozoa in the reproductive tract of sows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence of adding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteen multiparous sows were divided into 3 groups: group I (n= 5), insemina...

Semen is usually collected from bulls by use of an artificial vagina (AV) or by electroejaculation (EE). Semen collected by EE is often more dilute and may appear to be less motile than that collected by AV, although total sperm per ejaculate and sperm viability should be essentially similar (assuming the EE collection has been successful and complete). As fresh semen is very susceptible to env...

In this study, the quantitative and qualitative traits of semen were studied in seven rams of different breeds (Prolific Merino, Cokanski Tsigai, Barbados Blackbelly, Bábolna Tetra, Awassi, Ile de France and Suffolk), bred in Hungary. The semen parameters (density, volume, pH, mass motility, % motility, thawing and heat resistance), freezability of semen and the factors influencing these parame...

Introduction: Infertility is a common phenomenon in modern societies. Today, use of assisted reproductive technologies (ARTs) for treatment of infertility is common, and cryopreservation is one of these technologies. Cryopreservation has impact on the function and percentage of fertility of human sperm. In this study, motility, viability, nitric oxide, and DNA apoptosis were assessed before and...

Cryo banking of spermatozoa is an essential aspect of fertility preservation. With the advancements in cryobiology and better understanding of cryoprotectants and assisted reproduction, indications for semen banking are expanding. The exponential developments that have occurred over the years in the field of cryopreservation have proved that frozen sperm is as good as fresh sperm in fertilizing...

BACKGROUND Pregnancy rates with cooled equine semen can be unsatisfactory and show great variation. Information about first cycle pregnancy rates and pregnancy rates per cycle are often lacking from publicly available records. This retrospective cohort study was performed to evaluate the fertility of the Norwegian Coldblooded trotter. The aim of the study was to compare the breeding results aft...

The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, in vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30 degrees C or in liquid nitrogen vapour (LNV)]. For the in vivo trial, females were inseminated wit...

The screening and confirmatory tests available to a forensic laboratory allow evidence to be examined for the presence of bodily fluids. With the majority of evidence being submitted involving sexual assaults, it is important to have confirmatory tests for the identification of semen that are straightforward, quick, and reliable. The purpose of this study was to compare two commonly used semen ...

This study investigated the relationship between acrosin activation and pig sperm proacrosin binding protein (sp32) phosphorylation levels. Differently processed pig spermatozoa (fresh semen sperm, capacitation sperm, acrosome reaction sperm, capacitation-like sperm, and thawed sperm) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis. The fres...