The rates at which the sulfhydryl groups on each chain of hemoglobin fi4 react with N-ethylmaleimide and iodoacetate have been measured. The sulfhydryl group at position 93 reacts with both alkylating agents at approximately the same rate as normal liganded hemoglobin. The sulfhydryl group at position 112, which is not available in normal hemoglobin, reacts in hemoglobin f14 and does so much mo...

In studies of the oxidation of sulfhydryl compounds (I), selenite has been found to be an active catalyst. Considering the biological importance of sulfhydryl oxidations and the implication of sulfhydryl compounds in selenium poisoning (2-5), more information on selenite-catalyzed oxidations is desirable, This paper reports studies of the catalytic oxidation of GSH by selenite and its reaction ...

Pathological tau aggregation is one of the major hallmarks Alzheimer's disease, for which there are no effective treatments thus far. We previously reported that cinnamaldehyde, in cinnamon, could inhibit vitro by reacting with or both cysteines tau. Herein, we have investigated reaction kinetics and found cinnamaldehyde displays significant hyperreactivity towards compared to model sulfhydryl ...

The polyene antibiotic nystatin transforms the sugar-proton contransport system of Chlorella to a mere facilitated diffusion system. This experimental condition was used to test the sugar-translocating unit of the active uptake system for possible essential sulfhydryl groups. It could be shown that the catalyzed translocation of sugar is sensitive to the sulfhydryl-reactive compound N-ethylmale...

Peroxyacetyl nitrate reacts with the sulfhydryl groups of reduced glutathione. Thioester formation accounts for as much as 50% of the reacted sulfhydryl groups. As much as 60% of the reacted sulfhydryl groups can be accounted for as oxidized glutathione. The stoichiometry of the reaction is approximately 3 moles of sulfhydryl reacting per mole of peroxyacetyl nitrate, and this stoichiometry is ...

Chicken pepsinogen was extracted from the forestomach of chicken and purified by the consecutive application of acetone precipitation, chromatography on diethylaminoethyl cellulose, and gel filtration on Sephadex G-100. The purified pepsinogen was homogeneous on disc electrophoresis and sedimented as monodisperse material. Chicken pepsin was obtained by the activation of the pure zymogen at pH ...

1. The mechanism of succinic dehydrogenase action was studied by means of inhibitors. 2. The enzyme is inhibited by a large number of diverse compounds whose only common denominator appears to be their ability to react with SH groups. These compounds include quinonoid structures, sulfhydryl reagents, sulfhydryl compounds, copper, zinc, selenite, and arsenite. 3. In contrast to the above inhibit...

Disullide bonds in proteins and in seed meals were reduced with /3-mercaptoethanol to sulfhydryl groups. The generated sulfhydryl groups were then alkylated by treatment with 4-vinylpyridine. The cysteine residues were thus derivatized to S-(4-pyridylethyl)-L-cysteine residues. The cysteine derivative is stable to acid hydrolysis and on amino acid analysis by ion exchange elutes just before arg...

PURPOSE To investigate the role of externally facing membrane protein sulfhydryl groups in controlling lens permeability and transparency using the impermeant sulfhydryl complexing agent p-chloromercuriphenyl sulfonate (pCMPS). METHODS Membrane permeability changes were studied by measuring lens voltage and resistance. Ion movements were monitored by measuring 22Na+ and 45Ca2+ fluxes, and int...

We present the complete sequence of a cDNA encoding rabbit immunoglobulin kappa light chains of the Basilea isotype (K2). Although all rabbits seem to possess a K2 constant region gene, expression of this gene in most rabbits is minimal if present at all. Even in Basilea rabbits the majority of expressed immunoglobulins are of lambda type. We find that the sequence of our Basilea cDNA constant ...