Mammalian cell culture media used for the manufacture of therapeutic protein have advanced systematically from serum-containing into animal-free, protein-free and chemically defined formulations over the past decades. Initially driven by patient safety concerns associated with the use of animal-derived medium components, and later by inconsistent cell culture performance due to variability in p...

TASAKI, ICHIJI, IRWIN SINGER, AND AKIRA WATANABE. Excitation of squid giant axons in sodium-free external media. Am. J. Physiol, 2 I I (3) : 746-754. rg66.-Studies of membrane impedance and potential variation were made on squid giant axons under sodium-free external conditions. Intracellular perfusion by the double-cannulation method was employed. It was found that with favorable internal cond...

Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and safety concerns. The use of FBS in hybridoma culture media is examined here, ...

the purpose of this study was to examine the potential application of sorbitol, sucrose and gelatin in combination with glycerol in order to theileria annulata infected cell line cryo-preservation. the efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. as a result, the...

modifications in fat and fatty acid composition of silver carp that take place at each stage of canning process (precooking and sterilization) were evaluated. results showed, precooking led to decrease total fat and increase conjugated dines. the amounts of free fatty acids and thiobarbituric acids had no changed during precooking but these compounds like conjugated dine were increased after st...

analysis of non-laminar flows in coarse alluvial beds has a wide range of applications in various civil engineering, oil and gas, and geology problems. darcy equation is not valid to analyze transient and turbulent flows, so non-linear equations should be applied. non-linear equations are classified into power and binomial equations. binomial equation is more accurate in a wide range of velocit...

in this study, the effect of different sterilization treatments (115, 120 and 130 ° c, with same sterilization value) was evaluated on the chemical composition and lipid quality of canned silver carp. after sterilization, moisture and fat content were decreased and the amounts of protein and ash were increased, although significant differences due to the different thermal treatments were not ob...