This study compared SYBR Green real-time quantitative PCR (qPCR) with standard plate counting for the enumeration of Streptococcus mutans in oral samples. Oral samples (n = 710) were collected from high-caries-risk children for quantification of S. mutans by qPCR using primer pairs. The S. mutans copy number was calculated with reference to a qPCR quantification cycle (Cq) standard curve and co...

Cell densities of the fecal pollution indicator genus, Enterococcus, were determined by a rapid (3 h or less) quantitative polymerase chain reaction (QPCR) analysis method in 100 ml water samples collected from recreational beaches on Lake Michigan and Lake Erie during the summer of 2003. Measurements by this method were compared with counts of Enterococcus colony-forming units (CFU) determined...

The role of low-pressure RF plasma in the inactivation of Escherichia coli O157, Klebsiella pneumoniae, Proteus mirabilis, and Enterobacter sakazakii using N2-O2 and SF6 gases was assessed. 1×109 colony-forming units (CFUs) of each bacterial isolate were placed on three polymer foils. The effects of pressure, power, distance from the source, and exposure time to plasma gases were optimized. The...

In microbial food safety, molecular methods such as quantitative PCR (qPCR) and next-generation sequencing (NGS) of bacterial isolates can potentially be replaced by diagnostic shotgun metagenomics. However, the methods for pre-analytical sample preparation are often optimized for qPCR, and do not necessarily perform equally well for qPCR and sequencing. The present study investigates, through ...

die less explosive forming of circular plates is one of the most useful methods in the industries especially chemical applications. manufacturing of large vessel's head, multilayer rings and some parts of heat exchangers are the example of applying this method. many theoretical and experimental researches about examining the explosive forming of simple and single layer plate have been done...

In recent years, a lot of research has been done in the field of producing soft magnetic nano composites by means of mechanical alloying. These materials indicate more electrical resistance and permeability in comparison with soft magnetic composites (SMCs). In this research, reduction of silicothermal mixed powder (Nickel-oxide, Silicon and Iron) was carried out by mechanical alloying to form ...

Abstract: Dissolution and recovery of Mn-Al compacts with and without a chloride flux was studied by taking samples from the melt after addition of the compact. Events occurring after the addition of the compacts into the melt were studied using water quenched specimens after holding them for a specified time in molten state. The cross sections of these specimens were characterized by SEM a...

metal forming processes, compared with machining ones, reduce production steps and increasemanufacturing speed in addition to saving raw material. in this paper, forming process of column ofa steering mechanism is investigated by finite element analyses and experimental tests; andoptimum die design parameters are found. forming process parameters including die opening angle,bearing length, clea...

Metal forming processes, compared with machining ones, reduce production steps and increase manufacturing speed in addition to saving raw material. In this paper, forming process of column of a steering mechanism is investigated by finite element analyses and experimental tests; and optimum die design parameters are found. Forming process parameters including die opening angle, bearing length, ...

Quantification of bacteriophages by real-time quantitative PCR (qPCR) is an interesting alternative to the traditional plaque assay. Importantly, the method should in principle be able to discriminate between closely related phages that are indistinguishable by most other means. Here, a method is presented that employs qPCR to discriminate and quantify ten closely related lambdoid phages of Esc...