Dielectric relaxation (DR) of acrylodan-labeled human serum albumin (HSA/AC) was studied by phase-fluorometry. A non-monoexponential behavior of both the total fluorescence--and the DR decays has been found. The protein environment of the fluorescent marker shows DR times ranging from the pico to nanosecond timescale. In fluorescence emission decays measured on the red side of the fluorescence ...

Using front-surface fluorometry, we determined the effects of cocaine on force and cytosolic Ca2' concentration ([Ca2+] ) in the rat aorta. We also examined the effects of cocaine on 45Ca2` influx. Cocaine (10-` to 10` M) alone did not alter the resting level of [Ca2`], and force. Cocaine (<l0-` M), in a concentration-dependent manner, potentiated the 10-6 M serotonin (5-HT)-induced or 10` M no...

LEERS AND Lowa (1) HAVE PUBLISHED a method for the estimation of cholesterol on a micro scale in animal tissue by fluorometry involving the reaction of extracted cholesterol dissolved in trichloroethane with acetic anhydride and sulfuric acid. The present paper describes a simple and tested use of this reaction for the estimation of total cholesterol in 0.02 ml. of serum after extraction by a s...