The formation of hydrogen peroxide during the oxidation of NADH by purified preparations of cytochrome o has been demonstrated by employing three independent methods: polarographic, calorimetric, and fluorometric. The first two methods were used to assay for the accumulation of hydrogen peroxide and showed that hydrogen peroxide did accumulate as a product, but only about 30% of the oxygen cons...

There have been numerous studies on the distribution of endogenous histamine (1-5). Many mammalian tissues contain large to small amounts of histamine preformed. Amongst them skin, stomach and lung contain extremely high concentration of histamine. These organs are exposed to the outside directly or indirectly and prone to traumatic injury. The tissue level of histamine has been estimated with ...

      In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of antioxidant properties of plant extracts. The rate of the reaction was followed by monitoring the formation of DCF as a function of time. The antioxidant assay was carried out for different concentrations of gallic acid, Salvia ...

We describe the sensitive and precise determination 5-hydroxytryptophan in plasma from patients treated with this compound. The method is based on solvent extraction of plasma, high-performance liquid chromatography and continuous fluorometric detection after reaction with phthaldialdehyde in strong acid. The combined time for chromatography and reaction is 25 min. The sensitivity of the method...

The formation of hydrogen peroxide during the oxidation of NADH by purified preparations of cytochrome o has been demonstrated by employing three independent methods: polarographic, colorimetric, and fluorometric. The first two methods were used to assay for the accumulation of hydrogen peroxide and showed that hydrogen peroxide did accumulate as a product, but only about 30% of the oxygen cons...

The formation of hydrogen peroxide during the oxidation of NADH by purified preparations of cytochrome o has been demonstrated by employing three independent methods: polarographic, calorimetric, and fluorometric. The first two methods were used to assay for the accumulation of hydrogen peroxide and showed that hydrogen peroxide did accumulate as a product, but only about 30% of the oxygen cons...