glucose 6-phosphate dehydrogenase (g6pd) was purified from streptomyces aureofaciens and inactivated with butanedione and diethylpyrocarbonate. incubation of the enzyme with butanedione resulted in a rapid activity loss (80%) within 5 min, followed by a slow phase using a molar ratio to enzyme concentration of 100. fluorescence studies showed a conformational change in the butanedione-modified ...