The interpretation of a Raman spectrum is based on the identification of its characteristic molecular bands. However, the assignment of the vibrational modes is often compromised by the presence in the spectrum of an intense fluorescence background that covers the measured spectra. Several techniques have been employed to minimize the presence of this fluorescence in order to resolve and analyz...

Acute treatment with 300 mg/kg of pigmented guinea pigs with streptomycin sulfate induces an elevation of endogenous fluorescence in vestibular ampullary cristae. Fluorescence accumulates in all compartments of the epithelium, i.e., vestibular sensory and supporting cells and nerve fibers of the stroma and it was very intense 1 and 12 hours after its administration. Fluorescence decreased to co...

When the sub-core complex of photosystem II, D1/D2, is irradiated at 436 or 415 nm (absorption by chlorophyll and pheophytin and ß-carotene) or 540 nm (absorption primarily by pheophytin), the low temperature fluorescence spectrum has two maxima, at 685 and 674 nm. This shows the existence of at least two different fluorescent forms of chlorophyll (chlorophyll a and perhaps pheophytin a). When ...

Urinary 1-hydroxypyrene is a biomarker in the measurement of human exposure to polycyclic aromatic hydrocarbons. A rapid and simple derivative isopotential synchronous fluorescence method was developed for the direct determination of 1-hydroxypyrene in urine. A length of iso-intensity route was scanned on the three-dimensional fluorescence spectrum of urine and this result was combined with tha...

Abstract Raman spectra obtained by UV laser excitation above 260 nm are contaminated spectrally overlapping UV‐excited fluorescence. Here, we demonstrate a picosecond Kerr‐gated spectroscopy setup to suppress the fluorescence in excited 280 pulses. Specifically, use model sample that consists of acetonitrile small amount toluene for generating We were able acquire spectrum suppressing action 1‐...