Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of zoonotic food- and water-borne intestinal infections worldwide with clinical consequences ranging from mild diarrhoea to hemolytic uraemic syndrome. The genome of EHEC O157:H7 contains many regions of unique DNA that are referred to as O islands including the Shiga toxin prophages and pathogenicity islands encoding key virul...

Background: Virulence of S. typhi possessed an important factor for occurrence of typhoid fever in humans. Penetration of S. typhi in the intestinal mucosa is an important step in the establishment of infection because it allows microorganisms to pass through the epithelial barrier. This penetration is mostly determined by the motility of bacteria. Flagella are composed of a protein called flag...

Although Salmonella enterica serovar Typhimurium can undergo phase variation to alternately express two different types of flagellin subunit proteins, FljB or FliC, no biological function for this phenomenon has been described. In this investigation, we constructed phase-locked derivatives of S. enterica serovar Typhimurium that expressed only FljB (termed locked-ON) or FliC (termed locked-OFF)...

salmonella enterica is considered as one of the major pathogens in public health, worldwide. diseases caused by salmonella enterica serovars are especially prevalent in developing countries. it is one of the emerging pathogen in food-borne diseases, which is often found in contaminated chicken eggs. the aim of this study was to apply a pcr–rflp method using sau3ai and hhai restriction endonucle...

Fundamental aspects of outer membrane vesicle (OMV) biogenesis and the engineering producer strains have been major research foci for many in recent years. The focus this study was OMV production a variety Escherichia coli including wild type (WT) (K12 BW25113), mutants (from Keio collection) proprietary [BL21 BL21 (DE3)] strains. present investigated proteome prospective mechanism that underpi...

Malignant catarrhal fever (MCF) is a fatal disease of cattle that, in East Africa, follows contact with wildebeest excreting alcelaphine herpesvirus 1 (AlHV-1). Recently an attenuated vaccine (atAlHV-1) was tested under experimental challenge on Friesian-Holstein (FH) cattle and gave a vaccine efficacy (VE) of approximately 90%. However testing under field conditions on an East African breed, t...

The presence of cryptic fliC alleles in the genomes of 120 strains representative of the four Shigella species was investigated. One fragment was obtained by PCR amplification of fliC, with a size varying from 1.2 to 3.2 kbp, depending on the species or serotype. After digestion with endonuclease HhaI, the number of fragments in patterns varied from three to nine, with sizes of between 115 and ...

Worldwide, Dickeya (formerly Erwinia chrysanthemi) is causing soft rot diseases on a large diversity of crops and ornamental plants. Strains affecting potato are mainly found in D. dadantii, D. dianthicola and D. zeae, which appear to have a marked geographical distribution. Furthermore, a few Dickeya isolates from potato are attributed to D. chrysanthemi and D. dieffenbachiae. In Europe, isola...