Introduction Shake flask cultivation is nowadays a routine technique during process development for mammalian cell lines. During shaken culture, changes in agitation velocity, shaking diameter or shake flask size affect the hydrodynamics in the shake flask. This might be reflected in the growth of the cultured cells. Process parameters such as power input, mixing time, fluid velocity etc. have ...

A. 3-Amino-3-(hydroxymethyl)-1,5-dioxaspiro[5.5]undecane. A 1-L singlenecked, round-bottomed flask is equipped with an egg-shaped, Teflon®coated magnetic stirring bar (3.5 cm x 1.5 cm), capped with a rubber septum, flame-dried under vacuum, and cooled under an argon atmosphere (Note 1). After cooling to ambient temperature (21–23 °C), to the flask is added anhydrous N,N-dimethylformamide (DMF, ...

In a previous study a method for calibrating the loud deli­ vered by flask /tress springs was described. This method did not allow for changes in the mechanical properties of the springs when exposed to boiling water and spring compres­ sion. The effect of 20-25 cycles o f heal exposure and spring compression associated with flask press usage for the curing of denture base resin on the mechanic...

Conventional T-flasks with different sizes and layers are commonly used devices for static cell culture at laboratory scale. Due to their non-deformable structure and requirement of strict manufacture art, the rigid T-flask costs highly in production and transportation and, of course, produces more waste. In this study, we described a pentahedron shaped flexible flask made of a medical applicat...

INTRODUCTION Primary cells in vitro culture scale-up is a crucial issue in cell-based tissue and organ regeneration therapy. Reducing costs and space occupied by the cells cultured in vitro has been an important target. Cells cultured in vitro with the use of bioreactor with dextran microcarriers (Cytodex®) have potentially a chance to meet many of the cell therapy requirements. MATERIAL AND ...

introduction: the purpose of this study was to investigate the effect of flask investment materials on the color changes of different brands of resin denture teeth. methods: resin denture teeth (560) were divided into 2 groups according to the materials used for flask investment (silicone or gypsum). all the specimens were thermocycled between 4°c and 60°c with 60-s dwell times for 1,000 cycles...

Solubility and dissolution rate of drugs are of major importance in pre-formulation studies of pharmaceutical dosage forms. The solubility improvement allows the drugs to be potential biowaiver candidates and may be a good way to develop more dose-efficient formulations. Solubility behaviour of lamivudine, stavudine and zidovudine in individual solvents (under pH range of 1.2 to 7.5) was studie...

Solid wastes from agro-industries may be used as culture medium for enzymes synthesis by filamentous fungi. The brewer’s spent grain (BSG) is generated in abundance in the south of Brazil. Currently, most of this waste is used for animal feed. This work aims to extract and quantify the lipid presents in this residue. The lipids extraction was carried out by Soxhlet extraction method. Initially,...

In a retail company stock control is very important feature. Stock that done manually has many weaknesses especially for large companies transactions in day. This research aims to implement flask framework on the Monitoring application at PT. XYZ. Flask was chosen because of its easy use and lot libraries. The implementation this uses MVC architecture (Model, View, Controller). Then, it will be...