Despite being of high biotechnological relevance, many aspects of organic acid excretion in filamentous fungi like the influence of ambient pH are still insufficiently understood. While the excretion of an individual organic acid may peak at a certain pH value, the few available studies investigating a broader range of organic acids indicate that total organic acid excretion rises with increasi...

The readout of the genetic information of eukaryotic organisms is significantly regulated by modifications of DNA and chromatin proteins. Chromatin alterations induce genome-wide and local changes in gene expression and affect a variety of processes in response to internal and external signals during growth, differentiation, development, in metabolic processes, diseases, and abiotic and biotic ...

MAGGY is a gypsy-like LTR retrotransposon isolated from the blast fungus Pyricularia grisea (teleomorph, Magnaporthe grisea). We examined transposition of MAGGY in three P. grisea isolates (wheat, finger millet, and crabgrass pathogen), which did not originally possess a MAGGY element, and in two heterologous species of filamentous fungi, Colletotrichum lagenarium and P. zingiberi. Genomic Sout...

Gene replacement via homologous double crossover in filamentous fungi requires relatively long (preferentially >0.5 kb) flanking regions of the target gene. For this reason, gene replacement cassettes are usually constructed through multiple cloning steps. To facilitate gene function studies in filamentous fungi avoiding tedious cloning steps, we have developed a PCR-assisted DNA assembly proce...

Filamentous fungi and oomycetes are eukaryotic microorganisms that grow by producing networks of thread-like hyphae, which secrete enzymes to break down complex nutrients, such as wood and plant material, and recover the resulting simple sugars and amino acids by osmotrophy. These organisms are extremely similar in both appearance and lifestyle and include some of the most economically importan...

Reprogramming of a gene's expression pattern by acquisition and loss of sequences recognized by specific regulatory RNA binding proteins may be a major mechanism in the evolution of biological regulatory programs. We identified that RNA targets of Puf3 orthologs have been conserved over 100-500 million years of evolution in five eukaryotic lineages. Focusing on Puf proteins and their targets ac...

There is now a sufficient number of filamentous fungal genomes in the public databases to warrant at least initial comparisons with animal and plant genomes. Our interest lies in the control of multicellular morphogenesis, which is a feature of filamentous ascomycetes and basidiomycetes. Search of a representative collection of filamentous fungal genomes with gene sequences generally considered...

in the course of studies about fungi associated with pistachio (pistacia vera l.) die-back disease in the ghaen region of southern khorasan (iran), one species of geosmithia was found. the isolates were identified as geosmithia lavendula (raper & fennell) pitt based on its morphological and molecular properties. the genus geosmithia (ascomycota: hypocreales) is belonging to mitosporic filame...

The family Simuliidae is the host of simbiontes fungi that inhabit the digestive tracts of arthropods. This paper reports the presence of fungi in Simulium goeldii Cerqueira & Nunes de Mello larvae in Amazonia. We observed that the larvae are a good component of aquatic systems to isolate filamentous fungi.