The extent and nature of lymphocyte activation in the circulation in rheumatoid arthritis (RA) was investigated. Peripheral blood lymphocytes (PBL) from RA patients and healthy controls were separated into a number of discrete fractions by density in discontinuous Ficoll density gradients. Low density (activated) lymphocytes were found at significantly higher levels in RA, particularly in patie...

Puromycin aminonucleoside (PAN) has been suggested to reduce glomerular charge density, to create large glomerular "leaks," or not to affect the glomerular barrier. Therefore, we analyzed glomerular charge and size selectivity in vivo and in isolated kidneys perfused at 8 degrees C (cIPK) in control and PAN-treated rats. The fractional clearances (theta) for albumin and Ficoll of similar hydrod...

This is the first functional study of glomerular size and charge selectivity in mice. The aim was to investigate the controversial issue of glomerular permselectivity in animals exposed to glucosaminoglycan-degrading enzymes, hyaluronidase, and heparinase. Fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isoflurane anesthetized mice in vivo and in cooled isolated perf...

~ [ ~ H l F u c o s e (1.4pCi/mg of synaptosomal protein: sp. radioactivity 6.7Ci/mmol) and various concentrations of L-fucose were incubated with Ficoll-prepared synaptosomes. After lysis, fraction S was assayed for protein content and radioactivity. 33 nmol/mg of protein. Therefore under the conditions adopted in the present study the fucose turnover in synaptosomal fucosylglycoprotein appear...

T SHOULD BE POSSIBLE to separate cells of different types by utilizing differences in their specific gravities. Centrifugation of whole blood against high density fluids has been successful, at least in part, in separating leucocytes from peripheral blood.15 Gum acacia3 and bovine albumin1’2’5 have been used in this manner, but the method has been criticized because of the cost and labor involv...

Discontinuous Ficoll-sucrose gradients were used to prepare subcellular fractions from mouse cerebral cortex at various stages of postnatal development. Representative samples of each subcellular fraction were obtained by sedimentation in an analytical ultracentrifuge and each fraction was examined quantitatively and qualitatively by electron microscopy. The amount of synaptosomal material was ...

We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less...