Cyclic phosphatidic acid (cPA), an analog of lysophosphatidic acid (LPA), was previously identified in human serum. Although cPA possesses distinct physiological activities not elicited by LPA, its biochemical origins have scarcely been studied. In the present study, we assayed cPA formation from lysophosphatidylcholine in fetal bovine serum and found significant activity of transphosphatidylat...

background: pregnancy and accelerated fetal growth always are related with major metabolic changes and body fat redistribution and adiponectin is one of principle adipocyte hormones, so studying adiponectin changes during pregnancy may reveal some hidden parts of fetal metabolism. the aim of this study was to assess adiponectin and leptin levels in umbili cal cord and maternal serum, their rela...

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia. Fetuses are probably exposed to pain and/or discomfort, so the current practice of fetal blood harvesting is inhumane. Apart from moral concerns, sever...

Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell culture. A number of serum-free medium formula...

The constant growth of the population has pushed researchers to find novel protein sources. A possible solution this problem been found in cellular agriculture, specifically production cultured meat. In following review, key steps for vitro meat are identified, as well most important challenges. main biological and technical approaches taken into account discussed, such choice animal, animal-fr...

Ehrenwörtliche Versicherung Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet. I Abbreviations µM micromolar APS ammoniumperoxodisulfate BSA bovine serum albumin cDNA copy DNA cpm counts per minute C-terminal carboxyterminal dATP desoxyadenosintriphosphate dCTP desoxycytosintriphosphate DEPC diethylpyrocarbonate DNA desoxyribonucleicacid dNTP desoxynucleosidtriphosphate DTT...

Enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (SSCs) transplantation. Knock-out serum replacement (KSR) has been reported to enhance the proliferation of murine SSCs and human embryonic stem cells. The aim of the present study was to investigate the effect of KSR versus fetal bovine serum (FBS) ...

A photoacoustic correlation technique, inspired by its optical counterpart-the fluorescence correlation spectroscopy (FCS)-was tested for the first time, to our knowledge, to demonstrate the feasibility of low-speed flow measurement based on photoacoustic signal detection. A pulsed laser was used to probe the flow of light-absorbing beads. A photoacoustic correlation system of 0.8 s temporal re...

The scientific community is still looking for a bright, stable red fluorescent protein (FP) as functional as the current best derivatives of green fluorescent protein (GFP). The red FPs exploit the reduced background of cells imaged in the red region of the visible spectrum, but photophysical short comings have limited their use for some spectroscopic approaches. Introduced nearly a decade ago,...