In the hope of developing a vaccine against Clostridium difficile based on its toxin(s), we have developed a fermentation medium for the bacterium that results in the formation of Toxin A and contains no meat or dairy products, thus obviating the problem of possible prion diseases. Particular preparations of hydrolyzed soy proteins, especially Soy Peptone A3, have been found to replace both the...

A dextran/fructose mixture was obtained by fermentation of a sucrose rich media using Leuconostoc mesenteroides NRRL B512(f). The dextran and fructose mixture obtained by fermentation as well as a synthetic mixture of pure components were separated using simulated moving bed (SMB) chromatography. In the case of the synthetic feed, the purity in the raffinate (dextran) and extract (fructose) was...

Plackett-Burman experimental design was used to screen important medium components influencing the production of polyribosyl ribitol phosphate by Haemophilus influenzae type-B under submerged fermentation. Eleven media parameters such as Glutamic acid, Yeast extract, cystein, dextrose, NAD, heamin, NaCl, NH4Cl, Na2HPO4, KCl, and MgSO4.7H2O were screened in twelve experimental runs as per the de...

Lactic acid bacteria (LAB) are generally fastidious on artificiall media, but they grow readily in most food substrates and lower the pH rapidly to a point where competing organisms are no longer able to grow. Spontaneous fermentation of food/feed may differ from each other in terms of environmental microbiota and fermentable substrate. Spontaneous fermentation may fail, because it is not possi...

Dasamularista was prepared as per the national formulary to study the effect of container on the production of alcohol and the presence of microorganisms during the process of fermentation. Citraka (Plumbago rosea Linn.) which was one of the ingredient in the drug and subjected to "Sodhana" was also studied. The Citraka was impoverished by 50% with respect to plumbagin as a result of sodhana-pu...

A rapid carbohydrate oxidation microtube system (Carr Microbiologicals, Wichita, Kans.), designed for detecting the saccharolytic activity of gram-negative, nonfermenting bacilli, was evaluated and compared with the conventional oxidation-fermentation method. The oxidation of glucose, maltose, lactose, and xylose was tested with 430 strains of Pseudomonas, Acinetobacter, Achromobacter, Alcalige...

The utilization of food waste by microorganisms to produce omega-3 fatty acids or biofuel is a potentially low cost method with positive environmental benefits. In the present study, the marine microorganisms Thraustochytrium sp. AH-2 and Schizochytrium sp. SR21 were used to evaluate the potential of breadcrumbs as an alternate carbon source for the production of lipids under static fermentatio...

BACKGROUND Lignocellulosic materials are a diverse group of substrates that are generally scarce in nutrients, which compromises the tolerance and fermentation performance of the fermenting organism. The problem is exacerbated by harsh pre-treatment, which introduces sugars and substances inhibitory to yeast metabolism. This study compares the fermentation behaviours of two yeast strains using ...

A newly isolated fungus Aspergillus niger SOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation...